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pcDNA3.1 HA-rnSOX9

Plasmid

#62972

Purpose

Expresses HA-tagged rat SOX9

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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ETV2_pet28a

Plasmid

#131665

Purpose

Bacterial expression of ETS gene ETV2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV2 (ETV2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pWPI-TFDP1

Plasmid

#114299

Purpose

Constitutive expression of the TFDP1 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP1 (TFDP1 Human)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-otmyd88

Plasmid

#217485

Purpose

Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Rebl et al Cell Tissue Res. 2025 Oct 23. doi: 10.10

Insert

BFP-P2A-otPKRFL

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_FnIII-Notch

Plasmid

#188342

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_FnIII-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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MBD-IFP2.0C

Plasmid

#215748

Purpose

Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Expression

Mammalian

Promoter

SFFV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUASattB-PKA-SPARK

Plasmid

#106922

Purpose

Express PKA-SPARK, an activity reporter of protein kinase A in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

PKA-SPARK

Expression

Insect

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUASattB-ERK-SPARK

Plasmid

#106923

Purpose

Express ERK-SPARK, an activity reporter of ERK in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Expression

Insect

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Tensin2

Plasmid

#105298

Purpose

To study localization and function of Tensin2

Depositor

David Critchley, Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin2 (TNS2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

lacks N-terminal C1 domain

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP

Plasmid

#85438

Purpose

Lentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

S. typhi FliC

Use

Lentiviral

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
CAG-Otx2-IRES-GFPd2

Plasmid

#73996

Purpose

Plasmid expressing Otx2(isoform b)-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Otx2(isoform b)-Ires-GFP (Otx2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-NLS

Plasmid

#103840

Purpose

Flourescent marker for lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CFP-LacI-NLS

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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EF.mHES1.Ubc.GFP

Plasmid

#17625

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (Hes1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pXPR_212

Plasmid

#133457

Purpose

U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg

Plasmid

#159927

Purpose

Gene targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Insert

ERT2CreERT2

Use

Marmoset targeting

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-tdTomato

Plasmid

#106949

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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