We narrowed to 7,496 results for: addgene/1000
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Plasmid#206125Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation bindingDepositorAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only
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Cav2.1 HA EIA (E320A) pcDNA3
Plasmid#206124Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation bindingDepositorAvailable SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Jam1-AP-His
Plasmid#71952PurposeExpresses the extracellular region of the JAM-1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His
Plasmid#99746PurposeExpresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.DepositorInsertStx3 (STX3 Human)
Tagsmyc-myc-hisExpressionMammalianMutationMade six (6) silent mutations to confer resistanc…PromoterCMV/TOAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CNTF-HA
Plasmid#195517PurposeExpresses HA-tagged CNTF in mammalian cellsDepositorInsertCiliary neurotrophic factor (CNTF Human)
UseAAVTagsHA-tag and NGF signal peptideExpressionMammalianPromoterUbC promoter with beta-globin intronAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK4
Plasmid#172615PurposeExpresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-5xA
Plasmid#91992PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2844
Plasmid#143636PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
1-63 bcr/abl pcDNA3
Plasmid#27479DepositorExpressionMammalianMutationContains aa 1-63 of bcr and the complete abl chim…Available SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM3
Plasmid#72210PurposeMammalian expression of aa 227-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
TagsFLAGExpressionMammalianMutationΔ 1-226PromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND3
Plasmid#172623PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM1
Plasmid#72208PurposeMammalian expression of aa 1-372 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
TagsFLAGExpressionMammalianMutationΔ 373-860PromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM2
Plasmid#72209PurposeMammalian expression of aa 1-517 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
TagsFLAGExpressionMammalianMutationΔ 518-860PromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM4
Plasmid#72211PurposeMammalian expression of aa 373-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
TagsFLAGExpressionMammalianMutationΔ 1-372PromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCL2-pcw107-V5
Plasmid#64630Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826)_FIH
Plasmid#99508PurposeBacterial expression of hydroxylated Hif1a TAD (776-826)DepositorTagsHis6GB1ExpressionBacterialPromoterT7Available SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM5
Plasmid#72212PurposeMammalian expression of aa 518-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
TagsFLAGExpressionMammalianMutationΔ 1-517PromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:bmp4-pPH:vsvged
Plasmid#111157PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInserthBmp4 (BMP4 Human)
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianMutationwtPromoterCMVAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα2(I)-G610C
Plasmid#119837PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagsmCeruleanExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1 VAMP2 R125TAG
Plasmid#69877Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2DepositorInsertVAMP2 (Vamp2 Rat)
UseSynthetic BiologyExpressionMammalianMutationAmber and Ochre stop codons in the former linker …Available SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only