167,785 results

1. pcDNA3.4_17G6-1 light chain

   Plasmid

   #198250

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 17G6-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 17G6-1 (mouse kappa)
   

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-BI

   Plasmid

   #126475

   Purpose

   High-copy number vector for the co-expression of two genes in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVTNT-T7-KPNA1

   Plasmid

   #26677

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 1 (KPNA1 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   KPNA1 sequence contains a reported SNP at S73N

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK449 (BromoTag-mClover-BSD)

   Plasmid

   #214379

   Purpose

   BromoTag-mClover for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BromoTag-mClover-BSD
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CDK9 gRNA (BRDN0001162576)

   Plasmid

   #77170

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK9

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK9 (CDK9 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Wnt3A

   Plasmid

   #35908

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt3A (WNT3A Human)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Pur-CAG-hM4Di-mCherry

   Plasmid

   #80947

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM4Di-mCherry
   

   Tags

   mcherry fusion

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMDC32-HPB

   Plasmid

   #32078

   Depositor

   Fumi Katagiri

   Article

   Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

   Type

   Empty backbone

   Use

   Gateway destination binary vector

   Tags

   HA-Prescission-Biotin

   Promoter

   35S promoter

   Available Since

   Dec. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA Flag ERbeta

   Plasmid

   #35562

   Depositor

   Harish Srinivas

   Article

   Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

   Insert

   Estrogen Receptor beta (ESR2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   March 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-3XFLAG-EGFP-OMP25

    Plasmid

    #83354

    Purpose

    For tagging mitochondria with Sigma FLAG epitopes

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XFLAG-EGFP-OMP25
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3n1 (572)

    Plasmid

    #70727

    Purpose

    BetI/PhlF/SrpR BCD7 3-node v41-1

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Inserts

    srpR-ssrA
    betI-ssrA
    phlF-ssrA
    

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSiM24-eGFP

    Plasmid

    #67883

    Purpose

    Plant Gene expression Vector pSIM24 containing eGFP reporter

    Depositor

    Indu Maiti

    Article

    Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

    Insert

    eGFP
    

    Expression

    Plant

    Promoter

    M24

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET GFP LIC cloning vector (u-msfGFP)

    Plasmid

    #29772

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    SiriusGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-APEX2-C Cloning Vector

    Plasmid

    #128146

    Purpose

    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    Flag-APEX2

    Expression

    Mammalian

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-sgRNA-GFP

    Plasmid

    #200068

    Purpose

    For the knock down of GFP with an astrocyte specific mCherry expression

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    U6-sgRNA-GFP
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    GfaABC1D-mCherry

    Promoter

    U6, GfaABC1D

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-HA-USP36

    Plasmid

    #22579

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP36 (USP36 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHCMV-SS-antiFLAGscFv-TM

    Plasmid

    #198303

    Purpose

    Displays anti-FLAG scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiFLAGscFv-TM
    

    Expression

    Mammalian

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-Tre3G-29xGGGGCC-12xMS2

    Plasmid

    #99149

    Purpose

    Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

    Depositor

    Ron Vale

    Article

    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

    Insert

    29xGGGGCC 12xMS2 hairpins
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe puro HA PIK3CA H1047R

    Plasmid

    #12524

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1047R. Tumor-derived allele.

    Available Since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-hACE2

    Plasmid

    #145033

    Purpose

    Express human ACE2 with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    human ACE2 (ACE2 Human)
    

    Tags

    C9

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. Flag-HA-DUB3

    Plasmid

    #22593

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    DUB3 (USP17L9P Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV2)

    Viral Prep

    #105545-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA 3.2/V5-DEST MTFR1L-V5

    Plasmid

    #69822

    Purpose

    Expresses MTFR1L-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Mitochondrial fission regulator 1-like (MTFR1L Human)
    

    Use

    Retroviral

    Tags

    v5

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV_BiCHATe27_ChR2_mCherry (AAV PHP.eB)

    Viral Prep

    #213830-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_ChR2_mCherry (#213830). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Tags

    mCherry

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

25. pJC005.em

    Plasmid

    #182738

    Purpose

    CRISPR-Cas12a genome editing of E. faecium

    Depositor

    James Collins

    Article

    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pet26-MBP-ELIC

    Plasmid

    #39239

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    Eriwinia chrysanthemi - pentameric ligand gated ion channel
    

    Tags

    His-10 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLY100-RetroEFS-HER2-28BBz-WPRE

    Plasmid

    #192201

    Purpose

    Retro-HER2CAR

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-HER2CAR (ERBB2 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Mutation

    NA

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDONR221_SLC6A15

    Plasmid

    #131877

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A15 (SLC6A15 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pDY1314_RADARS_Luciferase

    Plasmid

    #193191

    Purpose

    Express Gaussia Luciferase conditional upon RNA transcript

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    RADARS Luciferase
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. TFORF2882

    Plasmid

    #143653

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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31. px330_Rosa_sgRNA

    Plasmid

    #97007

    Purpose

    Expresses Cas9 and Rosa26 locus specific sgRNA

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Insert

    Rosa26 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. pEF1α-Cre-2A-moxGFP

    Plasmid

    #207744

    Purpose

    Expression of the Cre recombinase linked to a moxGFP fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-moxGFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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33. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

    Plasmid

    #192204

    Purpose

    Lenti-GL(GFP-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-GL(GFP-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Mutation

    NA

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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34. Human SHIP1 (clone hp51CN)

    Plasmid

    #123347

    Purpose

    Expression of human SHIP1

    Depositor

    Christophe Erneux

    Article

    Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

    Insert

    INPP5D (INPP5D Human)
    

    Expression

    Mammalian

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pInt-ts

    Plasmid

    #66076

    Purpose

    The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    lambda integrase
    

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCherry-Sec61b-C1

    Plasmid

    #90994

    Purpose

    Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mCherry-Sec61b (SEC61B Synthetic, Human)
    

    Tags

    mCherry (red fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GPR68-Tango

    Plasmid

    #66371

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR68 (GPR68 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SMARCA2-GFP

    Plasmid

    #65390

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA2 (SMARCA2 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AiP15079 - pAAV-AiE0127m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5079)

    Plasmid

    #230403

    Purpose

    AiE0127m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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