We narrowed to 50,409 results for: Son

1. pHR Clover-VISH2

   Plasmid

   #188629

   Purpose

   Fluorescent reporter for SLP76/VISH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   VISH2
   

   Use

   Lentiviral

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-FLAG-Sestrin2-L261A

   Plasmid

   #73671

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

   Insert

   Sesn2 (SESN2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L261A changed Leucine 261 to Alanine

   Promoter

   CMV

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLEX_307_ms2_p65_hsf1

   Plasmid

   #79374

   Purpose

   Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   ms2-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SAFB1-pcDNA3.1

   Plasmid

   #32742

   Depositor

   Steffi Oesterreich

   Article

   Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

   Insert

   SAFB1 (SAFB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSC101-GFPmut2-mScarlet-I TAG

   Plasmid

   #208188

   Purpose

   nonsense mistranslation, TAG stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TAG*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSC101-GFPmut2-mScarlet-I TGA

   Plasmid

   #208189

   Purpose

   nonsense mistranslation, TGA stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSC101-GFPmut2-mScarlet-I TGATC

   Plasmid

   #208190

   Purpose

   nonsense mistranslation, TGA stop variant 2

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7TCT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC101-GFPmut2-mScarlet-I T7

   Plasmid

   #208191

   Purpose

   frameshift mistranslation, T7

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6TTTTTTT

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC101-GFPmut2-mScarlet-I A5

   Plasmid

   #208192

   Purpose

   frameshift mistranslation, A5

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAAA

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSC101-GFPmut2-mScarlet-I A4

    Plasmid

    #208193

    Purpose

    frameshift mistranslation, A4

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6AAAA

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSC101-GFPmut2-mScarlet-I A6T

    Plasmid

    #208194

    Purpose

    frameshift mistranslation, A6T

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6AAAAAAT

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSC101-GFPmut2-mScarlet-I C3T

    Plasmid

    #208195

    Purpose

    frameshift mistranslation, C3T

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6CCCT

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSC101-GFPmut2-mScarlet-I 6Trp7Ser

    Plasmid

    #208186

    Purpose

    mistranslation GFP and mScarlet-I positive control with Trp insertion

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Insertion 6Trp7Ser

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDB038a

    Plasmid

    #99211

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 29 and 184 of gIII

    Promoter

    Ppsp and PproK

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p5E-QUASM1

    Plasmid

    #155114

    Purpose

    5' entry vector containing QUASM1 promoter element.

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1
    

    Use

    Gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGagMBPHis

    Plasmid

    #42176

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    Gag protein sequence is from HIV-1 clone NL4-3
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pJ211_WT_low

    Plasmid

    #128848

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEO100c

    Plasmid

    #50550

    Purpose

    Expresses sfGFP under the PompC1157 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pDB038b

    Plasmid

    #99212

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 29, 83, and 184 of gIII

    Promoter

    Ppsp and PproK

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSETB-de4GFP

    Plasmid

    #85472

    Purpose

    Inducible expression of dual emitting pH sensitive GFP in bacterial cells

    Depositor

    S. James Remington

    Article

    Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

    Insert

    de4GFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S65T/C48S/H148C/T203C

    Promoter

    T7

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-N1-ro2GFP

    Plasmid

    #82370

    Purpose

    Expression of redox sensitive GFP in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LaM8-AK74

    Plasmid

    #159594

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAMP-CY1_NF1 T2 (+minintr)

    Plasmid

    #119205

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 2 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDB007ns2a

    Plasmid

    #99214

    Purpose

    Accessory plasmid for PACE of PylRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and Ptet

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 2 gRNA concatmer

    Plasmid

    #84879

    Purpose

    Empty concatmer vector in which 2 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pDB070.iodo.5

    Plasmid

    #99397

    Purpose

    Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PN25, Ptet, and PproK

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Use

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA6-HA-AML1-ETO

    Plasmid

    #12508

    Depositor

    Dong-Er Zhang

    Article

    Peterson et al Blood. 2007 May 15;109(10):4392-8. Epub

    Insert

    HA-AML1-ETO (RUNX1T1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

29. YFP-Rac2(V12)

    Plasmid

    #11397

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glycine 12 changed to Valine.

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAMP-CY1_NF1 T1 (+minintr, KDR)

    Plasmid

    #119200

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 1 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. DGK alpha

    Plasmid

    #35404

    Depositor

    Stephen Prescott, Robert Lefkowitz

    Article

    Nelson et al Science. 2007 Feb 2;315(5812):663-6.

    Insert

    DGK alpha (DGKA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pRK5-FLAG-Sestrin2-E451A

    Plasmid

    #73673

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pME-Fv2E-flt4um200-ns

    Plasmid

    #67696

    Purpose

    middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    Fv2E-flt4um200
    

    Use

    Tags

    HA

    Expression

    Mutation

    um200, no stop codon; N1056S

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEF SYT1-HaloTag

    Plasmid

    #197281

    Purpose

    Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EF1alpha

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p5E-mcs2

    Plasmid

    #138362

    Purpose

    Gateway compatible 5' entry clone contains mutli-cloning sites sequence2.

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    sequence multi cloning sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMiniT:SEP-beta2 TKIT donor

    Plasmid

    #169436

    Purpose

    Donor DNA for TKIT knockin of SEP to Gabrb2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SiT-EGFP

    Plasmid

    #166943

    Purpose

    mammalian expression of N' Sialyl Transferase tagged with EGFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Cell. 2008 Jun 13;133(6):1055-67. doi: 1

    Insert

    Sialyl Transferase N-terminus
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRSETB-ro2GFP

    Plasmid

    #82366

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/S65T/Q80R/S147C/Q204C

    Promoter

    T7

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDB023f1

    Plasmid

    #99218

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD4] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid

    #67279

    Purpose

    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    

    Use

    Tags

    linked to FlpO through an P2A ribosomal skipping …

    Expression

    Mammalian

    Mutation

    Promoter

    pdx

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge