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pSLQ17940-PHR-SFFV-NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Plasmid

#252646

Purpose

Human expression vector containing SFFV promoter, ABE8e fused to PYL1 and pH intein

Depositor

Stanley Qi

Article

Chen et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Use

Lentiviral

Promoter

SFFV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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JAB2144

Plasmid

#190344

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP Myc

Plasmid

#59757

Purpose

encodes multiple copies of an epitope tag Myc

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _Myc

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_Long_G_31AACTdel

Plasmid

#237350

Purpose

Expression plasmid for RSV Long G

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV Long G from Genbank Accesion AY911262.1

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_Long_F

Plasmid

#237349

Purpose

Expression plasmid for RSV Long F

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV Long F from Genbank Accesion AY911262.1

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-puro FLAG SREBP2

Plasmid

#32018

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pLZRS-IRES-deltaNGFR

Plasmid

#72930

Purpose

retroviral expression with FACS-selectable marker (extracellular part of NGFR)

Depositor

Esther van de Vosse

Article

van_de_Vosse et al Blood. 2009 May 7;113(19):4548-55. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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APEX2 - Tubulin in pEGFP

Plasmid

#66171

Purpose

APEX2 fused to the N-terminus of alpha tubulin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - alpha tubulin

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Cre-6xHis

Plasmid

#62939

Purpose

Expression of Cre-6xHis in bacterial of cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cre recombinase

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

Plasmid

#168806

Purpose

PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ASCL1-P2A-DLX2-T2A-mCherry

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP Δvpu/env

Plasmid

#101345

Purpose

Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta vpu/Env

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-Flex-TCB

Plasmid

#48332

Purpose

Cre-dependant TVA-mCherry fusion

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry

Use

AAV

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A2_G_31AACTdel

Plasmid

#237352

Purpose

Expression plasmid for RSV A2 G

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A2 G human codon optimized from Genbank Accesion KT992094.1

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DIO-hM4Di-mCherry

Plasmid

#81008

Purpose

Cre-dependent expression of hM4Di-mCherry under CBA promoter

Depositor

Bernardo Sabatini

Article

Hou et al Cell. 2016 Sep 22;167(1):73-86.e12. doi:

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

mCherry

Promoter

CBA

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMH-HA/Joseph2-Multitagged Plasmid

Plasmid

#226726

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pMH-HA/Joseph2

Tags

HA, Myc, GFP, Flag, V5, His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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L1_CsR-Ck1

Plasmid

#136124

Purpose

L1 in position 1 (next to LB in L2 construct), for CsR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:mALS_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-eNOS-GFP

Plasmid

#22444

Depositor

William Sessa

Article

Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

Insert

WT eNOS-GFP

Tags

GFP

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pMXs-Oct3/4

Plasmid

#13366

Purpose

Retroviral expression of mouse Oct3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLexM-SLC7A11

Plasmid

#197993

Purpose

Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC7A11 (SLC7A11 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Connexin43-GFP-APEX2

Plasmid

#49385

Purpose

Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Connexin43 - EGFP - APEX2

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG and linked by IRES

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYIC

Plasmid

#18673

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Type

Empty backbone

Tags

3Myc-EYFP-HA-His6 and ECFP-HA-His6

Expression

Mammalian

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCsA

Plasmid

#136067

Purpose

Acceptor plasmid for even level position 1, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-PGK-WP1-CRY2PHR-VP64

Plasmid

#171758

Purpose

Lentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

WP1-CRY2PHR-VP64

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCk3_spacer

Plasmid

#136073

Purpose

Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Tags

6X-His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pKOR1-mcs

Plasmid

#181755

Purpose

Allele replacement for Staphylococcus aureus

Depositor

Suzan Rooijakkers

Article

Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VAMP2

Plasmid

#42308

Depositor

Thierry Galli

Article

Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

Insert

VAMP 2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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CRY2-GFP-homer1c

Plasmid

#89442

Purpose

Localizes CRY2 to excitatory synapses

Depositor

Matthew Kennedy

Article

Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

Insert

CRY2-GFP-homer1c

Expression

Mammalian

Mutation

Contains Full length CRY2

Promoter

CAG

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pZS165 CRISPEY HH-HDV NotI

Plasmid

#114451

Purpose

Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Type

Empty backbone

Use

CRISPR; Cen/ars

Expression

Yeast

Promoter

ScGAL7p

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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HRE-doUnaG

Plasmid

#201713

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-DDX4N-mCh-Cry2WT

Plasmid

#101225

Purpose

DDX4(1-236) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

DDX4(1-236)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
tph-1(short)::rpl-22-HA

Plasmid

#122203

Purpose

C. elegans tagged ribosome expression plasmid

Depositor

Steven Flavell

Article

Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

Insert

tph-1(short)::rpl-22-HA

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCVhygro-F-G9a

Plasmid

#41721

Purpose

Retroviral expression of human G9a in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9a (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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