We narrowed to 32,507 results for: Eng;

1. MSCV-PIG-METTL14-WT

   Plasmid

   #141118

   Purpose

   Retroviral overexpression of WT METTL14 in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   METTL14 (METTL14 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV-PIG-METTL14-R298P

   Plasmid

   #141119

   Purpose

   Retroviral overexpression of mutant METTL14 in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   METTL14 (METTL14 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Substitution - Missense, position 298, R➞P

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGF_RT_FAST_Tq2CFP

   Plasmid

   #136981

   Purpose

   Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

   Depositor

   Klaus Theres

   Insert

   seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
   

   Expression

   Bacterial

   Mutation

   BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGGF_RT_FAST_Venus

   Plasmid

   #136980

   Purpose

   Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

   Depositor

   Klaus Theres

   Insert

   seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
   

   Expression

   Bacterial

   Mutation

   BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSFFV_sfCherry2_TagBFP

   Plasmid

   #83031

   Purpose

   Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry+full length_TagBFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   3 mutations in sfCherry full length: E118Q, T128I…

   Promoter

   SFFV

   Available Since

   Jan. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXTm-PH domain

   Plasmid

   #210326

   Purpose

   Expresses EGFP with PH domain

   Depositor

   Li Yu

   Article

   Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

   Insert

   PH domain
   

   Use

   Fish expression

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PC1-29-9-2_GAL4-VHL

   Plasmid

   #200434

   Purpose

   Expresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin Ligase

   Depositor

   Xue Gao

   Article

   Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

   Insert

   GAL4-VHL
   

   Tags

   GAL4, VHL

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PC17-605-9-1_BRD4-BD2-VPR

   Plasmid

   #200436

   Purpose

   Expresses the VPR transactivation domain linked to the BRD4(BD2) target protein

   Depositor

   Xue Gao

   Article

   Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

   Insert

   BRD4(BD2)-VPR
   

   Tags

   BRD4(BD2), VPR

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PC15-421-14-1_TRIM24-BD-VPR

    Plasmid

    #200435

    Purpose

    Expresses the VPR transactivation domain linked to the TRIM24(BD) target protein

    Depositor

    Xue Gao

    Article

    Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

    Insert

    TRIM24(BD)-VPR
    

    Tags

    TRIM24(BD), VPR

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXTm-zebrafish Tspan4a

    Plasmid

    #210327

    Purpose

    Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

    Depositor

    Li Yu

    Article

    Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

    Insert

    Tetraspanin 4a (tspan4a Zebrafish)
    

    Use

    Fish expression

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PC7-709-28-1_CRBN-VPR

    Plasmid

    #200437

    Purpose

    Expresses the VPR transactivation domain linked to the CRBN E3 Ubiquitin Ligase

    Depositor

    Xue Gao

    Article

    Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

    Insert

    CRBN-VPR
    

    Tags

    CRBN, VPR

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-G-Flamp1

    Plasmid

    #188567

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pL

    Plasmid

    #196286

    Purpose

    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. bNS2B47NS3

    Plasmid

    #155320

    Purpose

    co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PC3-709-33-10_GAL4-FKBP12(F36V)

    Plasmid

    #200438

    Purpose

    Expresses the GAL4 DNA Binding domain linked to the FKBP12(F36V) target protein

    Depositor

    Xue Gao

    Article

    Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

    Insert

    GAL4-FKBP12(F36V)
    

    Tags

    GAL4, FKBP12(F36V)

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RBD#4L

    Plasmid

    #193021

    Purpose

    hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

    Depositor

    Xue Gao

    Article

    Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

    Insert

    Cas13a
    

    Tags

    hnRNP C RRM

    Expression

    Bacterial

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RBD#3L

    Plasmid

    #193020

    Purpose

    hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

    Depositor

    Xue Gao

    Article

    Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

    Insert

    Cas13a
    

    Tags

    hnRNP A1 RRM2

    Expression

    Bacterial

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Dendra2-NS2B

    Plasmid

    #204218

    Purpose

    Expresses Dendra2-NS2B in Mammalian / Mouse Cell Lines

    Depositor

    Partha Mondal

    Article

    Aravinth et al Sci Rep. 2025 Mar 26;15(1):10358. doi: 1

    Insert

    Dengue (Dengue Type 2) NS2B (POLY Dengue Virus Serotype , Strain NGC)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC57-BBclone1

    Plasmid

    #137069

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ABE9

    Plasmid

    #194208

    Purpose

    Mammalian expression of ABE9

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q/L145T)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Promoter

    hSyn

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pM-CBE

    Plasmid

    #196293

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding CBE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-MbPylRS(DiZPK)

    Plasmid

    #91706

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

    Inserts

    pylS
    pylT
    

    Expression

    Mammalian

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-G-Flamp1-mut

    Plasmid

    #188568

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ABE8e-N108Q

    Plasmid

    #194157

    Purpose

    Mammalian expression of ABE8e-N108Q

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Td-CGBE

    Plasmid

    #196599

    Purpose

    Mammalian expression of Td-CGBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N46L); S. pyogenes Cas9 (D10A)

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV2-hSyn-G-Flamp1-mut

    Plasmid

    #188570

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    AAV

    Tags

    His tag

    Promoter

    hSyn

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PA-Dre

    Plasmid

    #169706

    Purpose

    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag-T2A-pMag-Dre247
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CALID

    Plasmid

    #169708

    Purpose

    Cre-activated light-inducible Dre recombinase

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. gNS2B47NS3 S135A

    Plasmid

    #155317

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S135A

    Promoter

    M13

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRGEB32-CrU6-2-35S

    Plasmid

    #226706

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrU6-2
    

    Use

    CRISPR

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRGEB32-CrActin-35S

    Plasmid

    #226708

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrActin
    

    Use

    CRISPR

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRGEB32-CrActin-UBI

    Plasmid

    #226709

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrActin
    

    Use

    CRISPR

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRGEB32-CrU6-2-UBI

    Plasmid

    #226707

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrU6-2
    

    Use

    CRISPR

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

    Plasmid

    #194751

    Purpose

    To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. eNS2B47NS3 S135A

    Plasmid

    #155318

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S135A

    Promoter

    M13

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pW34

    Plasmid

    #158210

    Purpose

    LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    LuxABCDE
    

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pM-ABE

    Plasmid

    #196292

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding ABE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pS-sg:GFP

    Plasmid

    #196296

    Purpose

    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pM-Cas9

    Plasmid

    #196325

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pM-Cas12a

    Plasmid

    #196291

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pS-cr:GFP

    Plasmid

    #196295

    Purpose

    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    

    Use

    CRISPR

    Tags

    SpeI-DR-BsaI-DR-SpeI

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. EF.GFP

    Plasmid

    #17616

    Depositor

    Linzhao Cheng

    Article

    Cui et al Blood. 2002 Jan 15. 99(2):399-408.

    Insert

    EF-1a promoter (EEF1A1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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