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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,602 results for: REP

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Showing: 5781 - 5800 of 31602 results

  6. pADC10-SETXhel

    Plasmid
    #193056
    Purpose
    Baculovirus expression of human Senataxin helicase domain for protein production
    Depositor
    Andrew Deans
    Article
    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:
    Insert
    Senataxin (SETX Human)
    Tags
    Flag
    Expression
    Insect and Mammalian
    Mutation
    codon optimized for insect cell expression
    Promoter
    hybrid p10 (insect) and CMV (human) expression
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    193056-plasmid-map-sequence-id-381199

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  7. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  13. pAAV-STAT-CA

    Plasmid
    #195576
    Purpose
    Expresses STAT3 constitutively active mutant
    Depositor
    Kevin Park
    Article
    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi
    Insert
    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    changed Alanine 662 to Cysteine and Asparagine 66…
    Promoter
    CMV enhancer and promoter
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195576-plasmid-map-sequence-id-383906

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  16. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

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Showing: 5781 - 5800 of 31602 results