We narrowed to 24,075 results for: Spr

1. lentiCRISPRv2_AMBRA1#1

   Plasmid

   #174152

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19_CRISPR_DpmrA

   Plasmid

   #160903

   Purpose

   CRISPR_Cas9, lambda red recombineering

   Depositor

   Anne-Catrin Uhlemann

   Article

   McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

   Inserts

   Cas9
   Lambda Red Recombineering Genes
   Zeocin Resistance Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR_Puro_sgTP53

   Plasmid

   #187819

   Purpose

   pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgTP53
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR_zeo_sgTP53

   Plasmid

   #183180

   Purpose

   pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgTP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. epiCRISPR

   Plasmid

   #135960

   Purpose

   Expresses SpCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPRv2_mSTING_scrambled_gRNA_2

   Plasmid

   #196628

   Purpose

   Scrambled control 2 for STING knock-out in murine cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING scrambled gRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti_SaCRISPR_GFP

   Plasmid

   #118636

   Purpose

   To clone sgRNA for SaCas9 (SauCas9)

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPRyl_MFE1

   Plasmid

   #84612

   Purpose

   Introduces DSB at MFE1 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPRyl_D17

   Plasmid

   #84611

   Purpose

   Introduces DSB at D17 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiCRISPRv2Cre

    Plasmid

    #82415

    Purpose

    Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    Cre Recombinase
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Mutated BsmBI cut site

    Promoter

    EFS (P2A)

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPRyl_XPR2

    Plasmid

    #84609

    Purpose

    Introduces DSB at XPR2 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. CE-8e-SpRY

    Plasmid

    #188913

    Purpose

    A-to-G base editor

    Depositor

    Xu Ma

    Article

    Cao et al Mol Ther Nucleic Acids. 2022 May 4;28:73

    Insert

    nSpRYCas9 ecTadA-8e
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiCRISPRv2_RB1#1

    Plasmid

    #174150

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPRv2_RB1#2

    Plasmid

    #174151

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nesprin3-C-18

    Plasmid

    #58194

    Purpose

    Localization: Nuclear Membrane , Excitation: N/A, Emission: N/A

    Depositor

    Michael Davidson

    Insert

    Nesprin3 (SYNE3 Human)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pL-CRISPR.SFFV.tRFP

    Plasmid

    #57826

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACRISPR-KM

    Plasmid

    #203587

    Purpose

    A sgRNA expression plasmid for genome editing in Burkholderia

    Depositor

    Leonilde Moreira

    Article

    Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge