We narrowed to 16,183 results for: GRN

1. pH-STEME-1-esgRNA

   Plasmid

   #138135

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
   

   Expression

   Plant

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. KAT5 sgRNA1

   Plasmid

   #138187

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT5

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT5 (KAT5 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. KAT5 sgRNA2

   Plasmid

   #138188

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT5

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT5 (KAT5 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPEPZ-sgRNAclone

   Plasmid

   #141090

   Purpose

   This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   p3

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LbCas12a-gRNA_BbsI_CAM

   Plasmid

   #183222

   Purpose

   LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HUWE1-sgRNA-1

   Plasmid

   #86924

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA1 (HUWE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

8. HUWE1-sgRNA-2

   Plasmid

   #86925

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA2 (HUWE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pSCB2-sgRNA

   Plasmid

   #129463

   Purpose

   Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   pgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 (SpeI)

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ULK1 sgRNA

    Plasmid

    #207559

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCAGCCAGGCCAGAAAGGTC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2_LSEC:Cas9green; erbb2_gRNA171

    Plasmid

    #199338

    Purpose

    pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    LSEC
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Chr7_Centromere-Targeting_gRNA

    Plasmid

    #195129

    Purpose

    dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr7 gRNA
    

    Expression

    Mammalian

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6_rd12_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232435

    Purpose

    pegRNA with optimized 3' modifications to correct the rd12 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6_rd12_nsgRNA(PP7)

    Plasmid

    #232436

    Purpose

    nsgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. ATG9A sgRNA

    Plasmid

    #207557

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CACTGAATACCAGCGCCTAG
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. M-ST1-sgRNA

    Plasmid

    #48672

    Purpose

    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    

    Use

    CRISPR

    Promoter

    hU

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Chr1q_Cassette-Integration_gRNA

    Plasmid

    #195126

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr1q gRNA
    

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid

    #187459

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_Cas9-hGem_gRNA2

    Plasmid

    #217659

    Purpose

    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA2 targeting the human AAVS1 safe harbour locus
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX459-sgRNA048_Grb10

    Plasmid

    #232934

    Purpose

    Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Grb10 S. pyogenes)
    

    Expression

    Mammalian

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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