We narrowed to 12,237 results for: LEA
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pPB-US-ECasE
Plasmid#83961PurposePiggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.DepositorInsertERT2-spCas9-ERT2
ExpressionMammalianMutationplease see depositor comments belowPromoterU6 (gRNA), EF1a (ERT2-Cas9-ERT2)Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BPK2101
Plasmid#65770PurposeBacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNADepositorInsertmammalian codon-optimized SaCas9, and SaCas9 gRNA
UseCRISPRTagsNLS-3xFlagExpressionBacterialPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit B3SE
Plasmid#49171PurposepHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
Tagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationGTG (V) to TGC (C)PromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3xFLAG-dCas9/pMXs-neo
Plasmid#51260PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGCDNsam-Hnf4a
Plasmid#33002DepositorInserthepatic nuclear factor 4 alpha (Hnf4a Mouse)
UseRetroviralAvailable sinceFeb. 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET MBP mCherry LIC cloning vector (MBP-mCherry)
Plasmid#29747DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-p38alpha
Plasmid#86832Purposemammalian expression of GFP-p38alphaDepositorAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCerulean-Rab11a-7
Plasmid#55390PurposeLocalization: Ras GTPase, Excitation: 433, Emission: 475DepositorAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-PGK-ABI-dCas9-P2A-mcherry
Plasmid#121513Purposeexpression of AB1-fused-dcas9 plasmidsDepositorInsertdCas9
UseLentiviralTagsAB1 and P2A-mcherryExpressionMammalianPromoterPGKAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pCMV-ABE7.8
Plasmid#102917Purposeexpresses ABE7.8 in mammalian cellsDepositorInsertABE7.8
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcAR7
Bacterial strain#52055PurposeStrain for use in Rinehart Lab Phosphoprotein synthesis kitDepositorBacterial resistanceNoneAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCl-HA-YAP
Plasmid#27007DepositorPublicationAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-SFFV-PYL1-sfGFP-Emerin
Plasmid#121532Purposeexpression of PYL-SfGFP-Emerin, lentivirus vectorDepositorAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-8
Plasmid#72347PurposeMammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1P-iCAG.copGFP
Plasmid#66577PurposeAAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENsDepositorInsertcopGFP
ExpressionMammalianPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT416
Plasmid#8607DepositorInsertbarnase, barstar
TagsphoA signal sequence to barnaseExpressionBacterialAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-myc-ERK2-MEK1_fusion
Plasmid#39194DepositorAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by
