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cYFP-pBSDONR_L1-L4

Plasmid

#247585

Purpose

Gateway donor vector carrying a C-terminal YFP tag, flanked by attL1 and attL4 sites for MultiSite Gateway recombination

Depositor

Juan Dong

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Aug 12;12

Insert

cYFP

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-HA-5'-CUX1

Plasmid

#244324

Purpose

5' AAVLINK plasmid for CUX1 expression

Depositor

Zhonghua Lu

Insert

CUX1 (CUX1 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCK071

Plasmid

#242907

Purpose

Constitutive expression of dCas9 and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-NADK-3xFLAG

Plasmid

#233888

Purpose

lentiviral overexpression vector for NADK

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-NADK (D184A)-3xFLAG

Plasmid

#233889

Purpose

lentiviral overexpression vector for NADK (D184A)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

D184A

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-Rap2A-3xFLAG

Plasmid

#233890

Purpose

lentiviral overexpression vector for RAP2A

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK (D184A)-3xFLAG

Plasmid

#233894

Purpose

lentiviral overexpression vector NADK (D184A)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

D184A

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Galt1-HT7-mGold

Plasmid

#230027

Purpose

Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-HaloTag7-mGold (B4GALT1 Synthetic, Human)

Tags

HaloTag,mGold

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#227773

Purpose

Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and tp1

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRAB14-HA

Plasmid

#214165

Purpose

Transient expression of RAB14

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

RAB14 (Rab14 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDY380

Plasmid

#182962

Purpose

Amp-resistant, low copy (p15A ori), E. coli 5'ptsI transcriptionally fused with GFP.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

ptsI

Use

Synthetic Biology

Tags

GFP

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDY390

Plasmid

#182963

Purpose

Amp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

ptsI

Use

Synthetic Biology

Tags

GFP

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBHRSF228

Plasmid

#135007

Purpose

MusF2 prenyltransferases from Nostoc sp. UHCC 0398

Depositor

Hideo Iwai

Article

Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

Insert

MusF2 prenyltransferase

Expression

Bacterial

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rab10

Plasmid

#109661

Purpose

Multisite gateway vector for 3' tagging with rab10

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab10 (rab10 Zebrafish)

Use

Zebrafish plasmids

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSP569

Plasmid

#139499

Purpose

Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl STL1 100 - 600

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab10

Plasmid

#109593

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab10

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab10 (rab10 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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JB48

Plasmid

#128930

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Cdc42GV-BirA*-HA-MAO (CDC42 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB57

Plasmid

#128931

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Cdc42TN-BirA*-HA-MAO (CDC42 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/C448Y with 5'UTR

Plasmid

#89318

Purpose

expression of C448Y spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87C448Y

Plasmid

#89329

Purpose

Inducible expression of M87 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP C448Y with 5'UTR

Plasmid

#89325

Purpose

Inducible expression of C448Y spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1C448Y

Plasmid

#89326

Purpose

Inducible expression of M1C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S46X with 5'UTR

Plasmid

#87729

Purpose

Expresses S46X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S46X

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S44L with 5'UTR

Plasmid

#87727

Purpose

Expresses S44L spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S44L

Promoter

pCMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/E112K with 5'UTR

Plasmid

#87723

Purpose

Expresses E112K spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

E112K

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag/L195V with 5'UTR

Plasmid

#87725

Purpose

Expresses L195V spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

L195V

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1E112K

Plasmid

#87715

Purpose

Expresses myc-M1E112K spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; E112K

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pVH004

Plasmid

#80401

Purpose

Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

CusR (cusR )
AraC (araC )
Anti-scaffold

Use

Synthetic Biology

Tags

C-terminal LZx domain

Expression

Bacterial

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH015

Plasmid

#80398

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold-ffGFP-3xFLAG
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal 3xFLAG tag and C-terminal LZx domain

Expression

Bacterial

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Has Service

Cloning Grade DNA

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-Golgi-7

Plasmid

#58100

Purpose

Localization: Golgi, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Golgi (B4GALT1 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
HA-TRIM28deltaLinker

Plasmid

#124958

Purpose

Mammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domains

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of linker region between CC domain and P…

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAW240

Plasmid

#170791

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

Plasmid

#134860

Purpose

Expresses EGFP-tagged recycling endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB11B (RAB11B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2 mBAX

Plasmid

#129577

Purpose

CRISPR deletion of murine BAX

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs murine BAX (Bax Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-EGFP-Cdc42(T17N)

Plasmid

#12601

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (T17N) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.Halo-LDLR

Plasmid

#162717

Purpose

Mammalian expression of human LDLR

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

LDLR (LDLR Human)

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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