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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 621 - 640 of 2493 results

  3. pSL690

    Plasmid
    #47753
    Purpose
    Expresses dCas9-VP64 fusion
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47753-plasmid-map-sequence-id-68212

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  4. SPACE-NG (pRZ5150)

    Plasmid
    #140244
    Purpose
    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPACE_NG
    Expression
    Mammalian
    Mutation
    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…
    Promoter
    CMV
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140244-plasmid-map-sequence-id-275728

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  8. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  9. mCherry-CAD

    Plasmid
    #73566
    Purpose
    Soluble red fluorescent activator of Orai1 channels
    Depositor
    Michael Cahalan
    Publication
    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
    Tags
    6x His and mCherry
    Expression
    Mammalian
    Promoter
    CMV Immediate Early
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73566-plasmid-map-sequence-id-137397

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  12. dCas9-CBP FL

    Plasmid
    #179550
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179550-plasmid-map-sequence-id-354775

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  14. pAC5-dual-dCas9VP48-sgTetO

    Plasmid
    #48237
    Purpose
    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9VP48 and sgTetO
    Use
    CRISPR
    Tags
    HA-tag and VP48
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48237-plasmid-map-sequence-id-69544

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  15. pCMV-eNme2-T.2-ABE8e

    Plasmid
    #185669
    Purpose
    Expresses eNme2-T.2-ABE8e in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-neNme2-T.2
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185669-plasmid-map-sequence-id-373132

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  16. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  17. pcDNA3-His:dCas9:nls:10P3

    Plasmid
    #135982
    Purpose
    Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9 fused to 10 copies of the P3 coiled-coil
    Use
    CRISPR
    Tags
    His
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135982-plasmid-map-sequence-id-308932

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  18. pcDNA3.1-CibN-dCas9

    Plasmid
    #60551
    Purpose
    Expresses CibN-dCas9 in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CibN-dCas9
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60551-plasmid-map-sequence-id-97050

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Showing: 621 - 640 of 2493 results