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We narrowed to 2,021 results for: D10

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Showing: 621 - 640 of 2021 results

  2. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139094-plasmid-map-sequence-id-273891

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  3. pET24a-ncOGT-FL

    Plasmid
    #190821
    Purpose
    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E.coli
    Promoter
    T7
    Available Since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190821-plasmid-map-sequence-id-379184

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  5. pH-ABE8e-spG

    Plasmid
    #200775
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9-spG(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200775-plasmid-map-sequence-id-399599

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  6. gTBEv3

    Plasmid
    #220619
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220619-plasmid-map-sequence-id-436981

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  10. pET-A3A(N57Q)-BE3

    Plasmid
    #158157
    Purpose
    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS
    Depositor
    Keith Joung
    Article
    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10
    Insert
    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    Tags
    6xHis tag on N-terminus
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    158157-plasmid-map-sequence-id-309498

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  11. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131300-plasmid-map-sequence-id-254651

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  13. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102885-plasmid-map-sequence-id-259794

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  15. pcDNA3.1-OGT_opt

    Plasmid
    #190858
    Purpose
    For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells.
    Promoter
    CMV
    Available Since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190858-plasmid-map-sequence-id-379243

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  18. pH-A3A-PBE

    Plasmid
    #119774
    Purpose
    Conversion of C to T in rice plants transformation
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-NLS-UGI-NLS
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119774-plasmid-map-sequence-id-257661

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  19. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102889
    Purpose
    PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102889-plasmid-map-sequence-id-198247

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Showing: 621 - 640 of 2021 results