166,511 results

1. mCherry-Fis1

   Plasmid

   #182580

   Purpose

   Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   mCherry-Fis1
   

   Use

   Tags

   Fis1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209652

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95K

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.CAG.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209654

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG PSAM4-5HT3 HC IRES EGFP

   Plasmid

   #119740

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F, R420Q, R424D, R428A

   Promoter

   CAG

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-ATR WT

   Plasmid

   #31611

   Depositor

   Aziz Sancar

   Article

   Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG PSAM4 GlyR IRES EGFP

   Plasmid

   #119739

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CAG

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1036 pcDNA3 Myr HA Akt1

   Plasmid

   #9008

   Purpose

   Mammalian expression of human Akt1 with myr, HA tags

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   HA and myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEv-Fat4-citrine

   Plasmid

   #112854

   Purpose

   Constitutive expression of human fat4 protein fused to citrine fluorescent protein

   Depositor

   David Sprinzak

   Article

   Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

   Insert

   FAT4 (FAT4 Human)
   

   Use

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

    Plasmid

    #160110

    Purpose

    Expresses murine Ifih1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ifih1 (Ifih1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. DTA in pCDEST2

    Plasmid

    #223705

    Purpose

    Mammalian expression vector encoding diptheria toxin A

    Depositor

    Rob Parton

    Article

    T. D. et al bioRxiv

    Insert

    Diphtheria toxin A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-Cytb5

    Plasmid

    #182579

    Purpose

    Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Cytb5
    

    Use

    Tags

    Cytb5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC57-CmYLCV

    Plasmid

    #205245

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234454

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100853

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV Retrograde and AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-hTERT-IRES-hygro

    Plasmid

    #85140

    Purpose

    Lentiviral expression of hTERT, used to create immortalized cell lines

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LifeAct-GFP-Fis1

    Plasmid

    #182576

    Purpose

    Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-Fis1
    

    Use

    Tags

    Fis1 and LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB-CMPE-ePPEplus

    Plasmid

    #205246

    Purpose

    For plant prime editing in wheat plants

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge