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We narrowed to 18,473 results for: SHE

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Showing: 1601 - 1650 of 18473 results

  10. pCMV/MASC_(GLuc)_INT

    Plasmid
    #68440
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68440-plasmid-map-sequence-id-124660

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  13. pU1/Sm/3' Box_(GLuc)_INT

    Plasmid
    #68438
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U1
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68438-plasmid-map-sequence-id-125562

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  14. RNA polymerase beta prime subunit FLAG

    Plasmid
    #102337
    Purpose
    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.
    Depositor
    Erin O'Shea
    Article
    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1
    Insert
    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    Tags
    3x FLAG with 3X GSGS linker
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102337-plasmid-map-sequence-id-197499

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  15. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid
    #68432
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68432-plasmid-map-sequence-id-125560

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  22. XW20

    Plasmid
    #65836
    Purpose
    Punc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65836-plasmid-map-sequence-id-120566

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  36. pJL1-MBP-DQNAT

    Plasmid
    #198215
    Purpose
    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    MBP_DQNAT
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198215-plasmid-map-sequence-id-393681

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  37. pJL1-PD-DQNAT

    Plasmid
    #198216
    Purpose
    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    PD-DQNAT
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198216-plasmid-map-sequence-id-393680

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  39. pENTR4 CDS A. thaliana PARP1

    Plasmid
    #175818
    Purpose
    pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Insert
    PARP1 (PARP1 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Mutation
    one silent mutation, annotated in GenBank file
    Promoter
    none
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175818-plasmid-map-sequence-id-346172

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  46. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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Showing: 1601 - 1650 of 18473 results