161,377 results

1. FUW-Luc-mCherry-puro

   Plasmid

   #183685

   Purpose

   Lentiviral vector expressing luciferase and mCherry.

   Depositor

   Andrew Kung

   Article

   Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

   Insert

   Luc-E2A-mCherry-T2A-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Human Ubiquitin C (UbC) promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDUAL CD81 (GFP)

   Plasmid

   #86980

   Purpose

   Lentiviral expression construct encoding CD81 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   CD81 (CD81 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV/hPGK

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn1-SIO-stGtACR2-FusionRed

   Plasmid

   #105677

   Purpose

   Expresses a soma-targeted GtACR2 in Cre-expressing neurons

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   stGtACR2
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. IRE1 alpha-pcDNA3.EGFP

   Plasmid

   #13009

   Depositor

   Fumihiko Urano

   Article

   Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

   Insert

   Inositol Requiring Enzyme-1 (ERN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. P2X4-pHluorin123

   Plasmid

   #52926

   Purpose

   express P2X4 protein with pHluorin at 123rd position

   Depositor

   Baljit Khakh

   Article

   Xu et al J Gen Physiol. 2014 Jul;144(1):81-104. d

   Insert

   mP2X4-pHluorin123
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCIP-Flag-TfebAA

   Plasmid

   #79014

   Purpose

   For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (Tfeb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S142A, S211A

   Promoter

   pCAGGs

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETCON-HtsptLB12v.3

   Plasmid

   #45121

   Depositor

   David Baker

   Article

   Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

   Insert

   HtsptLB12v.3
   

   Use

   Tags

   Aga2p for yeast surface display and c-myc epitope…

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-P2X2-YFP

    Plasmid

    #22400

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-NanoLuc

    Plasmid

    #189975

    Purpose

    Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Cloning

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6_HEK3+1T>A_(PP7-C4-Q1)

    Plasmid

    #232437

    Purpose

    pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV hSyn GFP-Cre

    Plasmid

    #175381

    Purpose

    expresses GFP-Cre under the control of a human synapsin promoter

    Depositor

    Jason Aoto

    Insert

    GFP-Cre recombinase
    

    Use

    AAV

    Tags

    Cre is fused to GFP. GFP is fused to additional n…

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-RSV-SpCas9

    Plasmid

    #85450

    Purpose

    Express SpCas9 in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pRSV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRSV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC.HA Vav2

    Plasmid

    #14554

    Depositor

    Joan Brugge

    Article

    Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. 478 p5Efli1ep

    Plasmid

    #31160

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    chimeric fli1ep enhancer/promoter fragment
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid

    #136348

    Purpose

    Lentiviral inducible expression (TetOn) of human BIRC5

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mRNA-CleanCap-mNeonGreen

    Plasmid

    #188248

    Purpose

    Template plasmid to transcribe mRNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti-EF1a-Ngn3ER-PuroR-mCherry

    Plasmid

    #197385

    Purpose

    To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    Ngn3ER-2A-PuroR-2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. T4 td upstream intron-PABP

    Plasmid

    #189972

    Purpose

    Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    T4 td upstream intron-PABP
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-flag-YTHDF2

    Plasmid

    #52300

    Purpose

    full length YTHDF2 with N-terminal flag tag for mammalian cell expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. HBA1 full 3'UTR-T4 td downstream intron

    Plasmid

    #189974

    Purpose

    Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    HBA1 full 3'UTR-T4 td downstream intron
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. AAV-mOXT-hM3Dq-mCherry-WPRE

    Plasmid

    #70717

    Purpose

    Expresses DREADDs Gq receptor under oxytocin promoter control

    Depositor

    Daniel Geschwind

    Article

    Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    mouse oxytocin

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLVX-Puro-TDP-43-Q331K

    Plasmid

    #133761

    Purpose

    Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed glutamine 331 to lysine.

    Promoter

    CMV

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-CaMKIIa-EGFP (AAV1)

    Viral Prep

    #50469-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    CaMKII

    Tags

    EGFP

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

28. M3-PAmcherry1

    Plasmid

    #114189

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Use

    Tags

    3xHA and PAmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pPB-cT3G-cERP2-HMGB2

    Plasmid

    #222532

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HMGB2 (HMGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIS2

    Plasmid

    #12177

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge