Skip to main content
Addgene
Showing: 1321 - 1360 of 2354 results

  3. CD4_LD 0_CS G_tTA-BFP

    Plasmid
    #58869
    Purpose
    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58869-plasmid-map-sequence-id-94230

    Enlarge

  4. pVH028

    Plasmid
    #80393
    Purpose
    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    Use
    Synthetic Biology
    Tags
    Fused by GS linkers to 4 SH3 domains
    Expression
    Bacterial
    Mutation
    Glycine 448 changed to Alanine, makes it primaril…
    Promoter
    Available since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80393-plasmid-map-sequence-id-166533

    Enlarge

  12. mCherry_LD 0_CS M_tTA-BFP

    Plasmid
    #58874
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    58874-plasmid-map-sequence-id-94235

    Enlarge

  13. mCherry_LD 0_CS A_tTA-BFP

    Plasmid
    #58873
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58873-plasmid-map-sequence-id-94234

    Enlarge

  15. pCUX1.1.0-gDNA

    Plasmid
    #112434
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1
    Depositor
    Kevin White
    Insert
    CUX1 (CUX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112434-plasmid-map-sequence-id-230375

    Enlarge

  29. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222015-plasmid-map-sequence-id-457427

    Enlarge

  32. GFP-FKBP-LBR

    Plasmid
    #222427
    Purpose
    To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR (LBR Human)
    Use
    Tags
    GFP-FKBP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    222427-plasmid-map-sequence-id-439259

    Enlarge

Showing: 1321 - 1360 of 2354 results