We narrowed to 44,315 results for: tra

Showing: 6921 - 6940 of 44315 results

Results per page:

Sort results by:

pMRE-Tn7-166

Plasmid

#118578

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV566 A-USF

Plasmid

#33360

Depositor

Charles Vinson

Article

Qyang et al Mol Cell Biol. 1999 Feb;19(2):1508-17.

Insert

USF (Usf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant Negative (see notes below)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pDEST27-Vinculin

Plasmid

#20144

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pDEST27-Vinculin (Vcl Mouse)

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pYX233-b2-DHFR

Plasmid

#163759

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2-DHFR (Dhfr Mouse, Budding Yeast)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

Plasmid

#127332

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PGL4-4X TE Down (GQs) Renilla

Plasmid

#70039

Purpose

Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

4x TE Down (G-quadruplexes) in 5'UTR

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Mutation

4 repeats of TE down motif (G-quadruplexes) in 5&…

Promoter

SV40

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pLenti.tie1.H2B.eYFP.W

Plasmid

#51293

Purpose

Lentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter

Depositor

Rusty Lansford

Article

Sato et al PLoS One. 2010 Sep 14;5(9):e12674. doi:

Insert

H2B-eYFP (H2BC21 Human)

Use

Lentiviral

Tags

H2B-eYFP

Promoter

mTie1

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-NHERF1-PDZ1-2

Plasmid

#28294

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 1-298 (deletion of the EB domain aa 2…

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
eTC GFP ß-actin ΔZip

Plasmid

#27124

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

This construct lacks β-actin 3′UTR and therefore …

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MED27

Plasmid

#15424

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED27 (MED27 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFUW-tetO- FOS

Plasmid

#125598

Purpose

doxycycline-inducible expression of human FOS in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Fos proto-oncogene, AP-1 transcription factor subunit (FOS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD233.7-3

Plasmid

#90955

Purpose

Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23114, PltetO-1, and PttrB185-269

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript-mouse Dicer

Plasmid

#50357

Purpose

Mouse Dicer cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

Dicer1 (Dicer1 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TRE-OKSM

Plasmid

#204498

Purpose

Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Inserts

OCT4 (POU5F1 Human)
KLF4 (KLF4 Human)
SOX2 (SOX2 Human)

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-dCas9-KRAB-GFP backbone

Plasmid

#194281

Purpose

Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

Plasmid

#187582

Purpose

Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SRSF7 (SRSF7 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GC52 pEGFPN1 anti-Gephyrin nanobody

Plasmid

#135224

Purpose

Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))

Tags

6xHis-HA-EGFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30 PTBP1 isoform1

Plasmid

#108591

Purpose

Recombinant His tagged protein production

Depositor

Chris Smith

Article

Wollerton et al RNA. 2001 Jun;7(6):819-32.

Insert

PTBP1 isoform 1 (PTBP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

isoform 1 (isoform c)

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge