167,601 results

1. pCM433kanT

   Plasmid

   #61262

   Purpose

   Sucrose counterselection (sacB) vector for unmarked allelic exchange.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Type

   Empty backbone

   Use

   Synthetic Biology; Sucrose counterselection

   Expression

   Bacterial

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTW334e

   Plasmid

   #237315

   Purpose

   expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

   Depositor

   Tina Wang

   Article

   Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

   Insert

   A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2
   

   Expression

   Bacterial

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Active Wnt1-V5

   Plasmid

   #43807

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   Wnt1 (WNT1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-DAAO

   Plasmid

   #60344

   Purpose

   Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

   Depositor

   Vsevolod Belousov

   Article

   Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

   Insert

   DAAO
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hsyn-GRAB_ATP1.0

   Plasmid

   #167577

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETM6-E12-vioABECD

   Plasmid

   #66537

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2+_HA-mAID-nanobody (Kless)

   Plasmid

   #117718

   Purpose

   mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   mAID-nanobody
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   codon-optimized and lysine-less mAID for murine e…

   Promoter

   SP6

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSBbi G418R mbIL21

   Plasmid

   #194329

   Purpose

   Consitiutive mammalian expression of membrane bound human IL-21

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   mbIL-21 (IL21 Human)
   

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCINeoFlag ALIX

   Plasmid

   #89859

   Purpose

   Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ADORA2A-Tango

    Plasmid

    #66210

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADORA2A (ADORA2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pWM_12x601_25bpLinker

    Plasmid

    #157788

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_25bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABE8.20-NG

    Plasmid

    #226857

    Purpose

    Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BL21ΔABCF

    Bacterial Strain

    #102270

    Purpose

    ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    Tetracycline

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

15. qTAG-AAVS1-PGK-Zeo

    Plasmid

    #227271

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

    Plasmid

    #213117

    Purpose

    Expression of a faster-running MinE mutant in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE(L4E)-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    L4E

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCYC1m_yeGFP

    Plasmid

    #64389

    Purpose

    encodes yeast enhanced GFP under control of minimal pCYC1 promoter

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    yeast enhanced GFP
    

    Expression

    Yeast

    Promoter

    pCYC1

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-ABCB1_STOP

    Plasmid

    #221424

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Insert

    ABCB1 (ABCB1 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

    Plasmid

    #213116

    Purpose

    Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    FRB-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1+-Tornado-nLuc

    Plasmid

    #212611

    Purpose

    For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-nLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge