We narrowed to 24,359 results for: nes

1. pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM)

   Pooled Library

   #198050

   Purpose

   By supplying an AAV cap gene of interest in trans, researchers can use this library to investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes

   Depositor

   George Church

   Article

   Jain et al bioRxiv 2023.01.31.526541

   Expression

   Mammalian

   Use

   AAV

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

2. DHFR Saturation Mutagenesis Library - Q33S TYMS

   Pooled Library

   #1000000196

   Depositor

   Kimberly Reynolds

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

3. DHFR Saturation Mutagenesis Library - R166Q TYMS

   Pooled Library

   #1000000195

   Depositor

   Kimberly Reynolds

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pRep-Cap Scanning Saturation Mutagenesis (SSM) pooled library

   Pooled Library

   #199601

   Purpose

   pRep-Cap SSM library enables VP1, VP2, and VP3 expression under the control of the endogenous p40 promoter

   Depositor

   George Church

   Article

   Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

   Expression

   Mammalian

   Species

   Aav2

   Use

   AAV

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

5. CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines

   Pooled Library

   #86538

   Depositor

   Jonathan Weissman

   Article

   Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines

   Pooled Library

   #86539

   Depositor

   Jonathan Weissman

   Article

   Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

   Pooled Library

   #136479

   Purpose

   Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

   Depositor

   Jonathan Weissman

   Article

   Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

8. Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

   Pooled Library

   #136478

   Purpose

   Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

   Depositor

   Jonathan Weissman

   Article

   Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

9. lentiCas9-Blast (Lentiviral Prep)

   Viral Prep

   #52962-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Promoter

   EFS-NS

   Tags

   None

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-GRAB_NEmut (AAV9)

    Viral Prep

    #123310-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NEmut (#123310). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NEmut plasmid DNA. hSyn-driven expression of GRAB-NEmut control norepinephrine sensor (does not respond to NE). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Promoter

    Syn

    Tags

    None

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-syn-Flpo (AAV Retrograde)

    Viral Prep

    #174378-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-syn-Flpo (#174378). In addition to the viral particles, you will also receive purified pAAV-syn-Flpo plasmid DNA. Syn-driven expression of the recombinase FLPo. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Promoter

    Tags

    None

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pLV_TRET_hNgn1_UBC_Bla

    Plasmid

    #61473

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn1 (NEUROG1 Human)
    Bla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV_TRET_hNgn2_UBC_Puro

    Plasmid

    #61474

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (NEUROG2 Human)
    Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-GRAB_NEmut (AAV Retrograde)

    Viral Prep

    #123310-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-GRAB_NEmut (#123310). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NEmut plasmid DNA. hSyn-driven expression of GRAB-NEmut control norepinephrine sensor (does not respond to NE). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Promoter

    Syn

    Tags

    None

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

16. pLV_TRET_Ngn2‐2A‐Ngn1

    Plasmid

    #61471

    Purpose

    3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (Neurog2 Mouse)
    Neurog1 (Neurog1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-fDIO-JRGECO1a (AAV1)

    Viral Prep

    #128317-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-JRGECO1a (#128317). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-JRGECO1a plasmid DNA. EF1a-driven, Flp-dependent expression of the biosensor jRGECO1a. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Promoter

    EF1a

    Tags

    None

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

18. pNF579

    Plasmid

    #26616

    Depositor

    Nancy Freitag

    Article

    Drevets et al Infect Immun. 2001 Mar . 69(3):1344-50.

    Insert

    gfpuv
    

    Use

    Tags

    gram-positive ribosome binding site

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCSaE500

    Plasmid

    #53182

    Purpose

    Inducible expression of E gene which produces a lysis protein that induces autolysis.

    Depositor

    Lingchong You

    Article

    Tanouchi et al Mol Syst Biol. 2012;8:626. doi: 10.1038/

    Insert

    pCSaE500
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    ampR-PampC cassette

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNN_v2

    Plasmid

    #32182

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer NN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge