161,398 results

1. pCMV-eNme2-T.1-ABE8e

   Plasmid

   #185668

   Purpose

   Expresses eNme2-T.1-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dimer 42 AA

   Plasmid

   #198198

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 42AA linker

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-42AA-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dimer 3 AA

   Plasmid

   #198197

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 3AA linker

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-3AA-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-mCherry

   Plasmid

   #114470

   Purpose

   mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcKv10.1

   Plasmid

   #85703

   Purpose

   mammalian expression Kv10.1

   Depositor

   Luis Pardo

   Article

   Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

   Insert

   KCNH1 (KCNH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP13790 - pAAV-AiE0475m-minBG-iCre(R297T)-BGHpA (Alias: CN3790)

   Plasmid

   #230384

   Purpose

   AiE0475m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCALNL-DsRed

   Plasmid

   #13769

   Purpose

   Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   DsRed2
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5-ActRIIA

   Plasmid

   #11754

   Depositor

   Jeff Wrana

   Article

   Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

   Insert

   Activin receptor IIA (Acvr2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. YIplac211-SEC7-mCherry2Bx6

   Plasmid

   #105267

   Purpose

   Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Sec7 portion (SEC7 Budding Yeast)
   

   Use

   Tags

   mCherry2Bx6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB5322

    Plasmid

    #204832

    Purpose

    An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW4 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p6sheLLr

    Plasmid

    #37318

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

    Insert

    HPV6L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pG3H-PE3max-attR1R2

    Plasmid

    #213049

    Purpose

    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Inserts

    Cas9 nickase
    M-MLV Reverse Transcriptase
    

    Use

    Note: this plasmid needs helper pvs1-vir2 for rep…

    Tags

    Expression

    Plant

    Mutation

    H840A, R240K, N413K,

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBT-PE2-PuroTK-pegRNA_GG

    Plasmid

    #173222

    Purpose

    piggyBac transposon containing both PE2 and a pegRNA cloning site.

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    PuroTK
    pegRNA cloning cassette
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, P2A, and hU6

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid

    #216154

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic exon and C-terminal FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSC234

    Plasmid

    #216357

    Purpose

    xhNup93-Nb3t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup93-Nb3t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSKI074

    Plasmid

    #11571

    Depositor

    Detlef Weigel

    Article

    Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

    Insert

    CaMV 35S enhancers
    

    Use

    Activation tagging

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. W118-1_hTGM2-V5

    Plasmid

    #180413

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-EGFP-RhoA-wt

    Plasmid

    #12965

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTET GFP10-FRB/FKBP-GFP11

    Plasmid

    #182497

    Purpose

    Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Inserts

    FRB
    FKBP (Fkbp1a Synthetic, Mouse)
    

    Use

    Tags

    split GFP10 tripartite and split GFP11 tripartite

    Expression

    Bacterial

    Mutation

    Promoter

    TET

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR-PIP-FUCCI

    Plasmid

    #118621

    Purpose

    Gateway cloning vector for the cell cycle indicator PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Gateway cloning

    Tags

    mCherry and mVenus

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLX-EF1a_Empty

    Plasmid

    #221480

    Purpose

    Empty vector control for pLX307 overexpresion experiments

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC5Kan-P2A

    Plasmid

    #51814

    Purpose

    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message

    Depositor

    Barry Ganetzky

    Article

    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:

    Insert

    P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
    

    Use

    Shuttle vector for insect expression vectors

    Tags

    Expression

    Mutation

    codons altered for Drosophila and to eliminate re…

    Promoter

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV14-3X-Flag-SARS-CoV-2 S

    Plasmid

    #145780

    Purpose

    Over expression of SARS-CoV-2 S (spike) in mammalian cell line

    Depositor

    Zhaohui Qian

    Article

    Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

    Insert

    SARS-CoV-2 S (S SARS CoV 2)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Codon optimized and C-terminal 19 amino acids del…

    Promoter

    CMV

    Available since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pAAV-pEF1a-EBFP-tTAn

    Plasmid

    #172122

    Purpose

    Constitutive expression of EBFP and rtTA-InteinN

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    EBFP, tTAn-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AnkyrinG-190-GFP

    Plasmid

    #31059

    Depositor

    Vann Bennett

    Article

    Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

    Insert

    Ankyrin G (Ank3 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGWB555

    Plasmid

    #74885

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. mSc-sspB

    Plasmid

    #114421

    Purpose

    Translocating to iLID domain

    Depositor

    Dorus Gadella

    Article

    Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

    Insert

    iLID domain
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RPL13-ECFP-SNAP

    Plasmid

    #187967

    Purpose

    expresses RPL13 with C-terminal tags ECFP and SNAP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    RPL13 (RPL13 Human)
    

    Use

    Tags

    ECFP-SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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