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pISlin41w(s) (lin-41 microRNA wt reporter)

Plasmid

#11906

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence (lin-41 Nematode)

Use

Luciferase

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-D614G-K417N-E484K-N501Y

Plasmid

#171750

Purpose

Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501Y

Depositor

Priyamvada Acharya

Article

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pISlin41m(s) (lin-41 microRNA mut reporter)

Plasmid

#11907

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence mut (lin-41 Nematode)

Use

Luciferase

Expression

Mammalian

Mutation

point mutations in miRNA complementary site

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-Quetzal-S-LA-WPRE

Plasmid

#253388

Purpose

Enhanced dendritic spine (via LifeAct) localization of a Cre-dependent GCaMP8m-mScarlet fusion with a rigid covalent linker

Depositor

Ofer Yizhar

Insert

Quetzal-S-LA

Use

AAV

Expression

Mammalian

Available Since

May 15, 2026

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN00H3-S-FLAG-His

Plasmid

#218038

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN00H3-S

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 128) C18/C23 S

Plasmid

#195033

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)

Expression

Mammalian

Mutation

C18/C23 S

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/30]

Plasmid

#206785

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/30 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/81]

Plasmid

#190556

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

Plasmid

#172105

Purpose

Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)-Gαs (GNAS Human)

Expression

Mammalian

Mutation

Gαs: C3S

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-027 mCherry(S-T)//Esp3I

Plasmid

#130229

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

mCherry

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

Plasmid

#72611

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine33, threonine34 and serine37 to ala…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pF(UG) hSyn NS34a-msGFP-'S-mito

Plasmid

#158768

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry

Plasmid

#133322

Purpose

Bacterial expression of TDP43 with FYW-S mutant CTD

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Bacterial

Mutation

All FYW in the TDP43 IDRs mutated to S

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry

Plasmid

#133321

Purpose

Bacterial expression of TDP43 with F-S mutant CTD

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Bacterial

Mutation

All F in the TDP43 IDRs mutated to S

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]

Plasmid

#133329

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All FYW to S in CTD of full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

Plasmid

#133328

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All F to S in CTD of full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

Plasmid

#133333

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All W to S in CTD of full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

Plasmid

#105182

Purpose

Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-ECFP (Cerulean) sponge control

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a S

Plasmid

#89388

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only