165,545 results

1. p3HA-hMIB1

   Plasmid

   #33317

   Depositor

   Vanessa Redecke

   Article

   Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

   Insert

   MIB1 (MIB1 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-bARGSer-AxeTxe

   Plasmid

   #192473

   Purpose

   Second-generation bacterial acoustic reporter gene derived from Serratia

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Inserts

   bARGSer
   AxeTxe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   the gene Ser39006_001280 was deleted from the clu…

   Promoter

   Native promoter from Enterococcus faecium and pBAD

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-Histag-Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-RO1.7-RPE65

   Plasmid

   #206346

   Purpose

   AAV plasmid expressing RPE65 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

   Insert

   Rpe65 (Rpe65 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   450-Met (C57BL6 natural variation)

   Promoter

   1.7kb human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-copGFP

   Plasmid

   #73030

   Purpose

   Express copGFP

   Depositor

   Kazuhiro Oka

   Insert

   copGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-PspCas13b-GEMSgRNA2

   Plasmid

   #204768

   Purpose

   pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   guide RNA targeting GEMS m6A sensor mRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Clathrin LC-15

   Plasmid

   #55019

   Purpose

   Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Clathrin LC (CLTB Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMU-NUCr

   Plasmid

   #61168

   Purpose

   Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   NLS-mCherry-GUS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-CMV-C-EGFP

   Plasmid

   #122844

   Purpose

   C-terminal EGFP tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Perox-RA

    Plasmid

    #209869

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Green Glifon600

    Plasmid

    #126207

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon600
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRMCD28

    Plasmid

    #167556

    Purpose

    Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

    Depositor

    Renato Morona

    Article

    Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

    Type

    Empty backbone

    Use

    Tags

    PhoA'

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYL-kasOp-FnCas12a3

    Plasmid

    #177875

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized the FnCas12a mutant EP16
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    kasOp*

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HTR7-Tango

    Plasmid

    #66415

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR7 (HTR7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-PLK2

    Plasmid

    #23691

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PLK2 (PLK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-coilin

    Plasmid

    #36906

    Depositor

    Greg Matera

    Article

    Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

    Insert

    coilin (COIL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hNET pcDNA3

    Plasmid

    #15475

    Depositor

    Randy Blakely

    Article

    Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

    Insert

    hNET (SLC6A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSV-CaMKII(1-290)

    Plasmid

    #45065

    Purpose

    Expression of constit active CaMKII

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    CaM Kinase II (Camk2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    truncated at amino acid 290

    Promoter

    RSV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWB411

    Plasmid

    #74805

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF2536

    Plasmid

    #142397

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX6 (SOX6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. VPS13B^GFP

    Plasmid

    #224588

    Purpose

    codon optimized GFP tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^GFP (VPS13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDUAL SRBI (GFP)

    Plasmid

    #86979

    Purpose

    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    SCARB1 (SCARB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT3-EF1a-Bmi1

    Plasmid

    #31783

    Depositor

    Xin Chen

    Article

    Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pME-mCherry-FLAG-CD59-GPI

    Plasmid

    #50378

    Purpose

    Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

    Depositor

    Reika Watanabe

    Article

    Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pQE-60NA-msGFP2

    Plasmid

    #135301

    Purpose

    Encodes a photostable monomeric superfolder GFP

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    msGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RKS-Fzd7/8 subtype NGS Wnt

    Plasmid

    #159628

    Purpose

    Express Fzd7/8 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd7/8 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 0_T7-mScarlet3

    Plasmid

    #225927

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDH-puro-CMV-VC3AI

    Plasmid

    #78907

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    VC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK642

    Plasmid

    #72391

    Purpose

    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)

    Depositor

    T. J. Kappock

    Insert

    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutated tyrosine-397 to phenylalanine to block ad…

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge