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We narrowed to 25,497 results for: crispr

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Showing: 721 - 740 of 25497 results

  2. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Publication
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    137729-plasmid-map-sequence-id-272646

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  5. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92062-plasmid-map-sequence-id-180159

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  10. pSL0828 (pQCascade_crRNA-4)

    Plasmid
    #130637
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130637-plasmid-map-sequence-id-255021

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  11. pDsRed-attP

    Plasmid
    #51019
    Purpose
    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    Use
    High copy, amp resistance
    Promoter
    3xP3 and None
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51019-plasmid-map-sequence-id-222558

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  13. lenti-sgRNA blast

    Plasmid
    #104993
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Publication
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104993-plasmid-map-sequence-id-342099

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  18. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid
    #67977
    Purpose
    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers
    Depositor
    Kosuke Yusa
    Publication
    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d
    Insert
    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    Use
    Lentiviral
    Mutation
    Deleted BbsI site within WPRE
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67977-plasmid-map-sequence-id-118694

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  19. pCMV-PEmax-P2A-GFP

    Plasmid
    #180020
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180020-plasmid-map-sequence-id-352752

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  20. xCas9(3.7)-BE3

    Plasmid
    #108380
    Purpose
    Mammalian expression vector for xCas9(3.7)-BE3
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.7)-BE3
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108380-plasmid-map-sequence-id-217617

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Showing: 721 - 740 of 25497 results