-
Plasmid#212746PurposeDoxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-NLS-VSV
Plasmid#212756PurposeDoxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo
Plasmid#212800PurposeConstitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cellsDepositorInsertE3(1)
UseTagsCUbo and MycExpressionMammalianMutationHOIP(C885A)PromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)
Plasmid#212814PurposeConstitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cellsDepositorInsertH2B
UseTags8xHis and CUbo(K63R)ExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-mCherry-FLAG
Plasmid#212798PurposeConstitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cellsDepositorInsertNUbo
UseTagsmCherry-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His7-NUbo-GFP
Plasmid#212791PurposeCopper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeastDepositorInsertHis7-NUbo-GFP
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo-GFP
Plasmid#212792PurposeCopper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeastDepositorInsertHis6-CUbo-GFP
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-CUbo-AID*-GFP
Plasmid#212796PurposeCopper-inducible expression of the artificial test substrate His6-CUbo-AID*-GFP in budding yeastDepositorInsertCUbo-AID*-GFP
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(63)-mCherry-VSV
Plasmid#212759PurposeConstitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-mCherry-VSV
Plasmid#212758PurposeConstitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-E3(63) I227A-VSV
Plasmid#212757PurposeCopper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)DepositorInsertE3(63) I227A
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPib1(I227A)PromoterCUP1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-NLS-VSV
Plasmid#212755PurposeConstitutive expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48)-mCherry-VSV
Plasmid#212749PurposeConstitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-mCherry-VSV
Plasmid#212748PurposeConstitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-NLS-VSV
Plasmid#212745PurposeConstitutive expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV
Plasmid#212740PurposeConsitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-mCherry-VSV and mycExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV
Plasmid#212739PurposeConsitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-mCherry-VSV and mycExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV
Plasmid#212737PurposeConsitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1)-CUbo-VSV
Plasmid#212736PurposeConsitutive expression of myc-E3(1)-CUbo-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-VSV and mycExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-myc-E3(1)-CUbo-VSV
Plasmid#212735PurposeConsitutive expression of myc-E3(1)-CUbo-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-VSV and mycExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-mCherry-VSV
Plasmid#212734PurposeConsitutive expression of CUbo-mCherry-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsmCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-mCherry-VSV
Plasmid#212731PurposeConsitutive expression of NUbo-mCherry-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsmCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-H2B-His8
Plasmid#212810PurposeConstitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cellsDepositorInsertH2B
UseTags8xHis and NUboExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-His6-NUbo-H2B-His8
Plasmid#212811PurposeConstitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cellsDepositorInsertH2B
UseTags6xHis-NUbo and 8xHisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-His8-H2B-CUbo
Plasmid#212812PurposeConstitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cellsDepositorInsertH2B
UseTags8xHis and CUboExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)
Plasmid#212813PurposeConstitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cellsDepositorInsertH2B
UseTags8xHis and CUbo(K48R)ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB399
Plasmid#203635PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertFB367+FB396
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB400
Plasmid#203636PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertFB367+FB397
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Cas9-nls-CDS1
Plasmid#160231PurposeCas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1DepositorInsertCas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
UseLevel 0 of moclo golden gate position cds1TagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::T48-Baz::mCherry
Plasmid#163927PurposeExpression of GrabFP-A-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4
Plasmid#163928PurposeExpression of GrabFP-A-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)
Plasmid#115432PurposeVector for expressing RPL28 with mutagenized exon2DepositorInsertsUseTagsExpressionYeastMutationexon 2 deleted, with MfeI / NheI cloning sitePromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS118 His14-Avi-28xGS-anti ALFA tag nanobody
Plasmid#199371PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-ALFA nanobody
UseTags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-COV M
Plasmid#165124Purposemammalian expression and localizationDepositorInsertCOV M (M codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6/sgClta-sfGFP-Clta
Plasmid#200261PurposeU6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.DepositorInsertsfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-FB-dCas9-puro
Plasmid#100547PurposeExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 proteinDepositorInsertsN-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin
UseCRISPRTagsFLAG, Biotin-acceptor-site (Avitag)ExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MYC-hTRAK2
Plasmid#225142PurposeExpresses MYC-tagged human TRAK2DepositorInsertTRAK2 (TRAK2 Human)
UseTagsMYCExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only