We narrowed to 22,787 results for: TES

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PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

Plasmid

#174620

Purpose

PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

Depositor

Volker Busskamp

Article

Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

Insert

enhanced green fluorescent protein

Use

Piggybac

Expression

Mammalian

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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MoFGFR3b-SV40PA

Plasmid

#86493

Purpose

mamalian cell expression of FGFR3b

Depositor

David Ornitz

Article

Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

Insert

FgfrR3 IIIb 12F5 (Fgfr3 Mouse)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/empty

Plasmid

#105505

Purpose

Empty backbone of MSCV-driven retroviral cDNA expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Type

Empty backbone

Use

Retroviral

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Promoter

pJ23119

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-FKBP-TACC3-shTACC3

Plasmid

#59354

Purpose

Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

EGFP and FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pmiCas9-msa

Plasmid

#164833

Purpose

miCas9-msa fusion protein expressing plasmid

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miCas9-msa

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

contains mSA sequence range is 5106-5447 and Brex…

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (N154S)-EGFP

Plasmid

#214150

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

Insert

STING (STING1 Human)

Use

Retroviral

Tags

EGFP

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHalo-MyosinIIA-C25

Plasmid

#128573

Purpose

Studies of cellular contractility

Depositor

Dylan Burnette

Article

Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

Insert

Myosin IIA (Myh9 Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA1-gRNA_A

Plasmid

#74374

Purpose

gRNA_A to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-TAF15N-mCherry-sspB

Plasmid

#122436

Purpose

Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

mCherry and sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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NSP4-WT-Flag

Plasmid

#210339

Purpose

Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CIB1-Myc

Plasmid

#32505

Depositor

John Groffen, Nora Heisterkamp

Article

Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

Insert

CIB1 (CIB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pLenti6.3/TO/V5-DEST-IGFBP2

Plasmid

#191006

Purpose

Lentiviral expression vector for IGFBP2

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Lentiviral

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM66

Plasmid

#65400

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM66 (TRIM66 Human)

Tags

GFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
EE-LRRK2

Plasmid

#193583

Purpose

Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

FYVE-CC2 and 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR221-FGFR4

Plasmid

#60531

Purpose

Entry vector for human FGFR4

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

FGFR4 (FGFR4 Human)

Use

Entry vector

Mutation

c2134a P712T

Promoter

NA

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
RCASBP(A)-3xFlag-mHoxd13wt

Plasmid

#145288

Purpose

Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

Hoxd13(wt) (Hoxd13 Mouse)

Use

Viral avian expression

Tags

3xFlag

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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A*24:07:01

Plasmid

#203283

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*24:07:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-p38 β/MAPK11 5'-UTR

Plasmid

#115357

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNA

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

p38 β/MAPK11 5'-UTR (MAPK11 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Dll4ICD-HPC4 pBacPAK8.3

Plasmid

#17335

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 4 (Dll4 Mouse)

Tags

HPC4

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
p8328 pLIX YAP1 S94A

Plasmid

#184529

Purpose

Expression of YAP1 S94A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S94A (YAP1 Human)

Use

Lentiviral

Mutation

S94A

Promoter

TRE promoter, Tet ON

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-myr-CSK-GFP

Plasmid

#83470

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

doxycycline-inducible promoter

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-SF2

Plasmid

#99020

Purpose

Bacterial expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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DNMT3L-dCas9

Plasmid

#126587

Purpose

DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

DNMT3L (DNMT3L Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3L-dCas9-NLS

Expression

Mammalian

Available Since

Dec. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

Plasmid

#185676

Purpose

SpCas9 and gRNA targeting the C-terminus of HsIRE1

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 gRNA (ERN1 Human)

Use

CRISPR

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only
pAdTrack-CMV HA ALAS-1

Plasmid

#22746

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

ALAS-1 (Alas1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only
GFP-SP140

Plasmid

#65394

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP140 (SP140 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-lox-roxSTOProx-tRFP-pA-lox

Plasmid

#196888

Purpose

Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

turbo (t)RFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

Plasmid

#62460

Purpose

expresses C151S and C288S double mutant human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteines 151 and 288 of Keap1 to respect…

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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