167,825 results

1. pFastBac HT JS-Rab10wt

   Plasmid

   #135557

   Purpose

   Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Tags

   6xHis tag, TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-7 asyn G51D

   Plasmid

   #105747

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

   Insert

   alpha-synuclein G51D (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   G51D

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SCP1-CMVenh-luc

   Plasmid

   #22216

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP1 (super core promoter 1)-CMVenh luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD4d-U6-1:white1-U6-3:white1

   Plasmid

   #84005

   Purpose

   pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

   Depositor

   Phillip Zamore

   Article

   Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

   Inserts

   white sgRNA-1
   white sgRNA-1
   

   Use

   CRISPR

   Promoter

   U6:1, U6:3, and dU6-1; dU6-3

   Available Since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYZ183

   Plasmid

   #98425

   Purpose

   Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYZ184

   Plasmid

   #98426

   Purpose

   Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINE-3C-mCherry

   Plasmid

   #214060

   Purpose

   Expression of fluorescent fusion proteins

   Depositor

   Ray Owens

   Insert

   mCherry
   

   Tags

   Hexahistidine

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-EGFP-C-IFT74

   Plasmid

   #218730

   Purpose

   Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

   Insert

   IFT74 (IFT74 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-syn-fRCaMP2

   Plasmid

   #125246

   Purpose

   Expresses red fluorescent calcium sensor in hippocampal slices

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRCaMP2
   

   Expression

   Mammalian

   Mutation

   D396A

   Promoter

   synapsin

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD434-SR mouse Alpha-synuclein

    Plasmid

    #89073

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pVITRO-HPV8 L1L2

    Plasmid

    #52598

    Purpose

    Expresses HPV8 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV8 L1
    HPV8 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid

    #214453

    Purpose

    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HC87 pEYFPN1 anti-Homer1 nanobody

    Plasmid

    #135223

    Purpose

    Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA-EYFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    pj23119

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-55:DMD-mEGFP

    Plasmid

    #159744

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_SPP1_WT_V5

    Plasmid

    #82981

    Purpose

    Gateway Donor vector containing SPP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    SPP1 (SPP1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS mouse IRS-2

    Plasmid

    #11372

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Expression

    Bacterial

    Available Since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pCLX-pTF-R1-DEST-R2-EBR65

    Plasmid

    #45952

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pVITRO1-BFP-CAAX

    Plasmid

    #184459

    Purpose

    Plasma membrane-targeted EBFP2 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    EBFP2-CAAX
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAC-dCLK

    Plasmid

    #33153

    Depositor

    Michael Rosbash

    Article

    McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

    Insert

    CLK (Clk Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pNB23

    Plasmid

    #103058

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Expression

    Bacterial

    Promoter

    hydrogen peroxide-inducible promoter katGp

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLX301-KANK1

    Plasmid

    #121985

    Purpose

    overexpress of KANK1

    Depositor

    GuangJun Zhang

    Article

    Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

    Insert

    KANK1 (KANK1 Human)
    

    Use

    Lentiviral

    Promoter

    CMV promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pKB01_mOrange2

    Plasmid

    #69102

    Purpose

    Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mOrange2
    

    Use

    Synthetic Biology

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDisplay-FRISZ

    Plasmid

    #176887

    Purpose

    Far-red fluorescent zinc indicator FRISZ in pDisplay

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    FRISZ
    

    Tags

    pDisplay backbone elements for export and membran…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMP4639

    Plasmid

    #107771

    Purpose

    Expression of EYFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EYFP in pME6010
    

    Tags

    pLacZ-EYFP

    Expression

    Bacterial

    Promoter

    pLacZ

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pUC-TALO8

    Plasmid

    #83409

    Purpose

    TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    TRP-ARS-8xLacO
    

    Expression

    Yeast

    Mutation

    Please see depositor comments below

    Promoter

    TRP1

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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