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Showing: 151 - 200 of 370 results

  1. R777-E174 Hs.PIK3R2-nostop

    Plasmid
    #70458
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70458-plasmid-map-sequence-id-125907

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  3. 1272 pBabe puroL Myr HA Akt3

    Plasmid
    #9019
    Depositor
    William Sellers
    Insert
    Akt3 (AKT3 Human)
    Use
    Retroviral
    Tags
    HA and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2005
    Availability
    Academic Institutions and Nonprofits only

  6. PIK3R2A

    Plasmid
    #42476
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIK3R2A (PIK3R2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. PIK3R2A

    Plasmid
    #42490
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIK3R2A (PIK3R2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. R777-E005 Hs.AKT3

    Plasmid
    #70289
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    70289-plasmid-map-sequence-id-125738

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  12. R777-E006 Hs.AKT3-nostop

    Plasmid
    #70290
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70290-plasmid-map-sequence-id-125739

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  13. pML31

    Plasmid
    #203343
    Purpose
    3 input AND circuit on pLW555 backbone: PTet-P1, PLux-A1, PIcaRA:PPhlF:PAmtR-Q2, PTac-I1
    Depositor
    Lauren Andrews
    Article
    Lebovich et al ACS Synth Biol. 2023 Aug 15. doi: 10.102
    Inserts
    phlF
    amtR
    qacR
    icaRA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PLux, PPhlF, PIcaRA, and PAmtR, PTac, and PTet
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203343-plasmid-map-sequence-id-400058

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  19. AKT3

    Plasmid
    #39038
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKT3 (AKT3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pJK429

    Plasmid
    #155376
    Purpose
    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    155376-plasmid-map-sequence-id-308997

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  24. pJK430

    Plasmid
    #155377
    Purpose
    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    155377-plasmid-map-sequence-id-309006

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  26. pKD184

    Plasmid
    #90953
    Purpose
    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively
    Depositor
    Jeffrey Tabor
    Article
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bba_J23114, PltetO-1, and PphsA
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90953-plasmid-map-sequence-id-178324

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  27. Halo-Tev-Akt3 WT-2X Flag

    Plasmid
    #87607
    Purpose
    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein
    Depositor
    Yimon Aye
    Insert
    Akt3 isoform 2 (AKT3 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    2 tandem flag tags and His-Halo-Tev
    Expression
    Mammalian
    Mutation
    Akt3 isoform 2
    Promoter
    SP6
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87607-plasmid-map-sequence-id-197919

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  28. pJK526

    Plasmid
    #155387
    Purpose
    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155387-plasmid-map-sequence-id-309008

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  31. pJK434

    Plasmid
    #155378
    Purpose
    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    155378-plasmid-map-sequence-id-324490

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  32. Halo-Tev-Akt3 C119S-2X Flag

    Plasmid
    #87608
    Purpose
    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of C119S mutant of human Akt3 protein
    Depositor
    Yimon Aye
    Insert
    AKT3 isoform 2 (AKT3 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    2 tandem flag tags and His-Halo-Tev
    Expression
    Mammalian
    Mutation
    cysteine 119 residue mutated to serine
    Promoter
    Available since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87608-plasmid-map-sequence-id-197921

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  33. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    155386-plasmid-map-sequence-id-309003

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Showing: 151 - 200 of 370 results