We narrowed to 7,530 results for: pcas

1. pCAG:ECFP

   Plasmid

   #32597

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

   Insert

   ECFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCas_2+5

   Plasmid

   #60594

   Purpose

   encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_2+5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCARSF63

   Plasmid

   #64695

   Purpose

   H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Type

   Empty backbone

   Use

   Tags

   CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGmRuby2smFPMyc

   Plasmid

   #71816

   Purpose

   encodes multiple copies of an epitope tag Myc ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Insert

   mRuby2_smFP_Myc
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG:Kaede

   Plasmid

   #39261

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

   Insert

   Kaede
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCas49

   Plasmid

   #82391

   Purpose

   rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   rbcL-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PccmK2

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGImC_Empty

   Plasmid

   #92015

   Purpose

   Empty backbone, includes IRES-mCherry

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMBPCas2/3

   Plasmid

   #89238

   Purpose

   Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas2/3
   

   Use

   Tags

   Strep II-MBP

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas1_Cas2/3

   Plasmid

   #89240

   Purpose

   Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas1 and Cas2/3
   

   Use

   Tags

   Strep II tag on Cas1

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAM12

    Plasmid

    #86471

    Purpose

    Expresses p12 and p9-mCitrine

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p12, p9-mCitrine
    

    Use

    Synthetic Biology

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaryP

    Plasmid

    #18945

    Depositor

    Michele Calos

    Article

    Groth et al Genetics. 2004 Apr . 166(4):1775-82.

    Insert

    phiC31 attP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAM17

    Plasmid

    #86473

    Purpose

    Expresses p12 and p9

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p12, p9
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. M-SPcas

    Plasmid

    #48668

    Purpose

    Mammalian S. pyogenes Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCasY1

    Plasmid

    #87687

    Purpose

    p15A plasmid expressing CasY1

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasY1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG:EYFP

    Plasmid

    #32596

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

    Insert

    EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

17. HeFSpCas9

    Plasmid

    #92355

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. HeFm2SpCas9

    Plasmid

    #92111

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    “Highly enhanced Fidelity” mut2 SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAM35

    Plasmid

    #86475

    Purpose

    Expresses p9-6His

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p9-6His
    

    Use

    Synthetic Biology

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCARSF85

    Plasmid

    #64694

    Purpose

    H6-TF-Smt3-GB1-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Use

    Tags

    CBD, HisTag, NpuDnaE-N102, Smt3, and TF

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge