We narrowed to 56,262 results for: plasmid

1. VE-cad-eGFP Donor plasmid

   Plasmid

   #92309

   Purpose

   Knock eGFP into human VE-cad locus

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad Homologous Recombination Arms (CDH5 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   about 30 a.a. truncated at end of protein

   Available Since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7 promoter + terminators reporter plasmid

   Plasmid

   #156456

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH-HA/Joseph2-Multitagged Plasmid

   Plasmid

   #226726

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pMH-HA/Joseph2
   

   Tags

   HA, Myc, GFP, Flag, V5, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCLIPf-Cox8A Control Plasmid

   Plasmid

   #101130

   Purpose

   Expression of CLIP-Tagged Cox8A in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   Cox8A (COX8A Human)
   

   Tags

   CLIP-tag (CLIPf)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pSL3927 (pDonor(plasmid)_Pse)

   Plasmid

   #200905

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (pTarget NGS)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CJ8421_04975 Rptr2 recording plasmid

   Plasmid

   #194211

   Purpose

   CJ8421_04975 Rptr2 targeted DNA sequence

   Depositor

   Chase Beisel

   Article

   Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

   Insert

   CJ8421_04975 Rptr2 DNA target
   

   Expression

   Bacterial

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEXP1/Joseph2-Multitagged Plasmid

   Plasmid

   #226724

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pEXP1/Joseph2
   

   Tags

   6xHis, HA, Myc, GFP, Flag, V5

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Plasmid L5 attB::Pleft* mCherry

   Plasmid

   #169411

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mCherry
   

   Use

   Unspecified

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Plasmid L5 attB:Psmyc double blue

   Plasmid

   #170050

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mTagBFP2
   CO BFP
   

   Use

   Unspecified

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCLIPf-H2B Control Plasmid

    Plasmid

    #101134

    Purpose

    Expression of CLIP-tagged Histone H2B in mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Histone H2B (H2BC11 Human)
    

    Tags

    CLIP-tag (CLIPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Plasmid L5 attB:Psmyc double green

    Plasmid

    #169412

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mWasabi
    mWasabi CO
    

    Use

    Unspecified

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Plasmid L5 attB:Psmyc triple red

    Plasmid

    #169413

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet proper
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Incoherent feedforward (IFF) plasmid

    Plasmid

    #169748

    Purpose

    Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV miRNA control plasmid

    Plasmid

    #31508

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCLIPf-NK1R Control Plasmid

    Plasmid

    #101125

    Purpose

    Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Neurokinin-1 Receptor, Truncated (TACR1 Human)
    

    Tags

    10xHis, CLIP-tag (CLIPf), and FLAG Epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMCP-GPI Control Plasmid

    Plasmid

    #101132

    Purpose

    Expression of MCP-Tagged GPI anchor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    GPI
    

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PAX6 CKO 2/7 Donor plasmid

    Plasmid

    #68467

    Purpose

    donor plasmid for targeting PAX6 locus

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    FRT flanked exon4 of PAX6 gene (PAX6 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    mouse PGK promoter

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACP-GPI Control Plasmid

    Plasmid

    #101131

    Purpose

    Expression of ACP-Tagged GPI anchor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    GPI
    

    Tags

    ACP-Tag (ACP wt)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACP-ADRβ2 Control Plasmid

    Plasmid

    #101128

    Purpose

    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Tags

    ACP-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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