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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 60,538 results for: Gene

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Showing: 1171 - 1200 of 60538 results

  6. pCS2TAL3-DD

    Plasmid
    #37275
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    Flag, Fok I DD, Tal-C, and Tal-N
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37275-plasmid-map-sequence-id-46872

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  8. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  9. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  12. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37276-plasmid-map-sequence-id-46873

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  14. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #162379-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV9)

    Viral Prep
    #35509-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid
    #84561
    Purpose
    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops
    Depositor
    Robert Singer
    Article
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    Use
    Lentiviral
    Tags
    Flag, Suntag, oxBFP, AID (auxin-inducible degron)
    Promoter
    UbC
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    84561-plasmid-map-sequence-id-331246

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  20. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available Since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

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  26. pAAV-CAG-tdTomato (codon diversified) (AAV2)

    Viral Prep
    #59462-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available Since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-CAG-tdTomato (codon diversified) (AAV5)

    Viral Prep
    #59462-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available Since
    Oct. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pAAV-hsyn-GRAB_5-HT1.0 (AAV9)

    Viral Prep
    #140552-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_5-HT1.0 (#140552). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_5-HT1.0 plasmid DNA. Synapsin-driven expression of GRAB_5-HT1.0 sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s
    Promoter
    Syn
    Available Since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  30. CMV-R-GECO1.2

    Plasmid
    #45494
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1.2
    Expression
    Mammalian
    Mutation
    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    45494-plasmid-map-sequence-id-123029

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Showing: 1171 - 1200 of 60538 results