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Showing: 1951 - 2000 of 4674 results

  1. pNHA_OROV_NSm

    Plasmid
    #208935
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSm
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 949 bp from 5' end (Gn), deleted 29…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208935-plasmid-map-sequence-id-419020

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  3. pNX24-RFP-MS2SL

    Plasmid
    #227819
    Purpose
    Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene
    Depositor
    Jeffrey Gerst
    Article
    Haimovich et al bioRxiv 2024.11.06.622258
    Inserts
    3xHA-RFP-24xMS2SL
    Neo resistance
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and SV40
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    227819-plasmid-map-sequence-id-469648

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  4. pCHA_SBV_NSs

    Plasmid
    #208938
    Purpose
    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 47 bp at 5' end and 510 bp from 3&#x…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208938-plasmid-map-sequence-id-419026

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  5. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72263-plasmid-map-sequence-id-134557

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  6. pCDH-EF1-DIO-copGFP

    Plasmid
    #72253
    Purpose
    Express copGFP under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72253-plasmid-map-sequence-id-131724

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  7. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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  8. pCDH-EF1-Nluc

    Plasmid
    #73024
    Purpose
    Express Nluc reporter under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73024-plasmid-map-sequence-id-133955

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  9. pEIAV-SIN6.1 CBGFPW

    Plasmid
    #44173
    Depositor
    John Olsen
    Insert
    enhanced green fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    chromophore mutations
    Promoter
    CB, CMV enhancer/chicken beta-actin promoter
    Available since
    May 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    44173-plasmid-map-sequence-id-59865

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  11. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  12. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  13. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  15. pENTR-L4R1-CAG

    Plasmid
    #139522
    Purpose
    Provides constitutive promoter between attL4-attR1 cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    CAG promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139522-plasmid-map-sequence-id-274624

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  16. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  17. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

    Enlarge

  18. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  35. GPKAnls

    Plasmid
    #61094
    Purpose
    GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal
    Depositor
    Steven Green
    Article
    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.
    Insert
    pkac2
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. GPKAnes

    Plasmid
    #61092
    Purpose
    GFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signal
    Depositor
    Steven Green
    Article
    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.
    Insert
    pkac2
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

    Enlarge

  40. pCDH-EF1-Nluc

    Plasmid
    #73024
    Purpose
    Express Nluc reporter under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73024-plasmid-map-sequence-id-133955

    Enlarge

  41. pEIAV-SIN6.1 CBGFPW

    Plasmid
    #44173
    Depositor
    John Olsen
    Insert
    enhanced green fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    chromophore mutations
    Promoter
    CB, CMV enhancer/chicken beta-actin promoter
    Available since
    May 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    44173-plasmid-map-sequence-id-59865

    Enlarge

  42. pLenti-EF1a-GFP-miRE_shRen-Puro

    Plasmid
    #135683
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135683-plasmid-map-sequence-id-327605

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  43. pLenti-EF1a-GFP-miRE_shRen-Blast

    Plasmid
    #135682
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135682-plasmid-map-sequence-id-327602

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Showing: 1951 - 2000 of 4674 results