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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,033 results for: pET

Showing: 1951 - 2000 of 15033 results

  1. pETM-60-NusA-PfAgo_F

    Plasmid
    #146270
    Purpose
    Bacterial Expression of PfAgo
    Depositor
    Elisa Izaurralde
    Insert
    PfAgo
    Use
    Tags
    Expression
    Bacterial
    Mutation
    one non silent mutation A907G (I303V) and one sil…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146270-plasmid-map-sequence-id-468111

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  2. pET28-Dnmt3a3L-sc27

    Plasmid
    #71827
    Purpose
    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.
    Depositor
    Albert Jeltsch
    Article
    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do
    Insert
    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71827-plasmid-map-sequence-id-254300

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  3. pETM-11-6His-DmBelle_B

    Plasmid
    #145950
    Purpose
    Bacterial Expression of DmBelle
    Depositor
    Elisa Izaurralde
    Insert
    DmBelle (bel Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145950-plasmid-map-sequence-id-407048

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  4. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid
    #122850
    Purpose
    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122850-plasmid-map-sequence-id-238544

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  9. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40787-plasmid-map-sequence-id-68902

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  12. pET-Bst-LF-Idaho

    Plasmid
    #170469
    Purpose
    Expresses His-tagged Bst-LF-Idaho in E. coli
    Depositor
    Jonathan Schmid-Burgk
    Insert
    Bst-LF-Idaho
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170469-plasmid-map-sequence-id-338354

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  16. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  30. pET28a-HaloTag7SS-DogTag

    Plasmid
    #171775
    Purpose
    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    HaloTag7SS-DogTag
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    DogTag inserted in HaloTag7 between residues D139…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171775-plasmid-map-sequence-id-338524

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  34. pET28a-His-MinD_MinE

    Plasmid
    #133621
    Purpose
    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
    Depositor
    Petra Schwille
    Article
    Loose et al Science. 2008 May 9;320(5877):789-92. do
    Insert
    His-MinD_MinE (minD, minE E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133621-plasmid-map-sequence-id-271463

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  43. LHP2691 - pET30-Ub-K63A

    Plasmid
    #32179
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K63A
    Promoter
    T7
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  44. LHP2690 - pET30-Ub-K48A

    Plasmid
    #32178
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K48A
    Promoter
    T7
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  47. pET28-Mff(1-61)-PP-GST

    Plasmid
    #73042
    Purpose
    Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Mff(1-61) (Mff Mouse)
    Use
    Tags
    GST-tag and PreScission protease site
    Expression
    Bacterial
    Mutation
    Truncation containing amino acids 1-61
    Promoter
    T7
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73042-plasmid-map-sequence-id-134011

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Showing: 1951 - 2000 of 15033 results