We narrowed to 31,602 results for: REP

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lenti-Cas9-VQR-GFP_activity_reporter

Plasmid

#87156

Purpose

This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

GFP and sgRNA targeting GFP (NGA restricted)

Use

Lentiviral

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-BFLF1

Plasmid

#162661

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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1kb Zfp521 promoter reporter

Plasmid

#104188

Purpose

luciferase reporter to assay Zfp521 promoter activation

Depositor

Kathryn Hentges

Article

Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

Insert

Zfp521 promoter - 1 Kb (Zfp521 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

none

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pISlin41w(s) (lin-41 microRNA wt reporter)

Plasmid

#11906

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence (lin-41 Nematode)

Use

Luciferase

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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TP901 GFP BAC Reporter

Plasmid

#78216

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 2xFLAG-2xSTREP EMI1

Plasmid

#158947

Purpose

Express human EMI1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

EMI1 (FBXO5 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFUGW-GFP-2-cut reporter

Plasmid

#131669

Purpose

Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

GFP-2-cut reporter

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMIR-REPORT-MYC CDS_WT

Plasmid

#108924

Purpose

MYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vector

Depositor

Jianjun Chen

Article

Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

Insert

MYC (MYC Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET22-Strep-TEV-Nsp10

Plasmid

#154958

Purpose

Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

Depositor

Justin Kollman

Insert

Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)

Tags

Strep-TEV

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA4/TO-mu24-ORF24-2xStrep

Plasmid

#138453

Purpose

Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24-ORF24 (ORF24 MHV68/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

mu24 amino acids 1-191 fused to ORF24 amino acids…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-ORF66-2xStrep

Plasmid

#130957

Purpose

Lentiviral vector for expression of KSHV ORF66 with a C-terminal 2xStrep tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

2xStrep

Promoter

CMV

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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PhiC31 GFP BAC Reporter

Plasmid

#78214

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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Bxbi GFP MCP Reporter

Plasmid

#78225

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir129-2-wt

Plasmid

#46730

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir129-2-wt (MIR129-2 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-HA-SrtAstrep (pKS78)

Plasmid

#50029

Purpose

Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Tags

HA

Expression

Yeast

Promoter

pGAL

Available Since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter mir30-wt

Plasmid

#46695

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-wt (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir30-5

Plasmid

#46692

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-5 (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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