161,670 results

1. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-GFAP-hM4D(Gi)-mCherry

   Plasmid

   #50479

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNCS-Antares

   Plasmid

   #74279

   Purpose

   Constitutive expression of Antares in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Antares
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EBFP2-N1

   Plasmid

   #54595

   Purpose

   Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pPB-cT3G-cERP2-CEBPD

   Plasmid

   #192897

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of CEBPD

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   CEBPD (CEBPD Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 (+) -myc-OGA(GS-544)C181

   Plasmid

   #193996

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pORTMAGE311B

   Plasmid

   #120418

   Purpose

   Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

   Depositor

   Csaba Pál

   Article

   Szili et al bioRxiv (2018)

   Inserts

   MutL E32K
   Lambda Beta
   XylS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   Pm

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. METTL7A pEGFP-N2 (890)

   Plasmid

   #62017

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   METTL7A (METTL7A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pNH-NanoLuc

   Plasmid

   #173075

   Purpose

   Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSG5-BMLF1

    Plasmid

    #72636

    Purpose

    Mammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulator

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BMLF1/BSLF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q157H

    Promoter

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pMaCTag-Z29

    Plasmid

    #120072

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJH643

    Plasmid

    #172168

    Purpose

    Reporter plasmid for pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sfGFP(N39*, N135*, Y151*)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3 Flag Jnk2a2

    Plasmid

    #13801

    Depositor

    Roger Davis

    Article

    Sluss et al Mol Cell Biol. 1994 Dec . 14(12):8376-84

    Insert

    Jnk2a2 (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pEPYC0CM0416

    Plasmid

    #154573

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Article

    Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

    Insert

    MinSyn_110
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPYC0CM0411

    Plasmid

    #154568

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Article

    Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

    Insert

    MinSyn_105
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSBbi-RP

    Plasmid

    #60513

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTK586

    Plasmid

    #114716

    Purpose

    SNAP-tagging at LGN's N-terminal locus (BSD)

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTK473

    Plasmid

    #114715

    Purpose

    sgRNA for LGN's N-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    LGN sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-pcDNA3.1 Puro-CAG-Voltron2

    Plasmid

    #172909

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge