167,910 results

1. AV04

   Bacterial Strain

   #115926

   Purpose

   Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pMpGWB107

   Plasmid

   #68561

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Citrine

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. VP64-dCas9

   Plasmid

   #177171

   Purpose

   VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   VP64-dCas9(D10A, H840A)
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xFLag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRS6E1b-luc(-732/-721) WT

   Plasmid

   #45383

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI-1 promoter -732/-721
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-Syn-Ace-mNeon2-Kv2.1PR

   Plasmid

   #195522

   Purpose

   Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   Ace-mNeon2-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Ace-mNeon 81S; SY linker

   Promoter

   Synapsin

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVRC8400-SARS-CoV-2-NTD-AVI

   Plasmid

   #160475

   Purpose

   Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

   Insert

   SARS-CoV-2-NTD
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human NCKX6-FlagTag (pcDNA3.1+)

   Plasmid

   #75217

   Purpose

   Mammalian expression of SLC8B1

   Depositor

   Jonathan Lytton

   Article

   Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

   Insert

   SLC8B1 (SLC8B1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1_puro_shNRF2-2

   Plasmid

   #230087

   Purpose

   shRNA silencing human NRF2 gene

   Depositor

   Xingguo Zhu

   Article

   Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

   Insert

   Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag Jnk1a2

   Plasmid

   #13751

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1a2 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

10. AAV_NLS-SaCas9-NLS-VPR

    Plasmid

    #68496

    Purpose

    AAV vector containing SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SaCas9-VPR
    

    Use

    AAV and CRISPR

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid

    #178057

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-FLAG
    

    Use

    AAV

    Tags

    FLAG-MBP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. VV073: 1xCox8 - GCaMP5G in fubi

    Plasmid

    #58509

    Purpose

    Expresses the calcium sensor GCaMP5G in the mitochondria

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - GCaMP5G
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-7 asyn A30P

    Plasmid

    #105726

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A30P (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    A30P

    Promoter

    T7

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-GFP-WT1 +KTS N terminal HA

    Plasmid

    #217639

    Purpose

    Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 +KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-GFP-WT1 -KTS N terminal HA

    Plasmid

    #217641

    Purpose

    Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 -KTS isoform (WT1 Human)
    

    Use

    Gateway cloning

    Tags

    HA

    Mutation

    p.408_410delKTS

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5sheLL

    Plasmid

    #46953

    Purpose

    encode HPV5 L1 and L2, Bicistronic, too large to self-package

    Depositor

    John Schiller

    Article

    Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

    Insert

    HPV5L1+L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. XLone-BSD SPI1 P2A mCherry

    Plasmid

    #140031

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-4591NES

    Plasmid

    #138377

    Purpose

    Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-ROCK
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 28A_APEX2_MSP1D1

    Plasmid

    #170923

    Purpose

    Express APEX2_MSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

    Insert

    APEX2-MSP
    

    Expression

    Bacterial

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSG5-EGFP

    Plasmid

    #49246

    Purpose

    encodes enhanced green fluorescent protein (EGFP)

    Depositor

    Rebecca Riggins

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

22. HPRT

    Plasmid

    #22884

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Mutation

    PGK, NeoR, and BGH polyA are between the two geno…

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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23. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFastBac HT JS-Rab10wt

    Plasmid

    #135557

    Purpose

    Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Tags

    6xHis tag, TEV site

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pT7-7 asyn G51D

    Plasmid

    #105747

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

    Insert

    alpha-synuclein G51D (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    G51D

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SCP1-CMVenh-luc

    Plasmid

    #22216

    Depositor

    James Kadonaga

    Article

    Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

    Insert

    SCP1 (super core promoter 1)-CMVenh luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCFD4d-U6-1:white1-U6-3:white1

    Plasmid

    #84005

    Purpose

    pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Inserts

    white sgRNA-1
    white sgRNA-1
    

    Use

    CRISPR

    Promoter

    U6:1, U6:3, and dU6-1; dU6-3

    Available Since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pYZ183

    Plasmid

    #98425

    Purpose

    Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYZ184

    Plasmid

    #98426

    Purpose

    Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOPINE-3C-mCherry

    Plasmid

    #214060

    Purpose

    Expression of fluorescent fusion proteins

    Depositor

    Ray Owens

    Insert

    mCherry
    

    Tags

    Hexahistidine

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pD434-SR mouse Alpha-synuclein

    Plasmid

    #89073

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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34. pVITRO-HPV8 L1L2

    Plasmid

    #52598

    Purpose

    Expresses HPV8 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV8 L1
    HPV8 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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35. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid

    #214453

    Purpose

    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HC87 pEYFPN1 anti-Homer1 nanobody

    Plasmid

    #135223

    Purpose

    Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA-EYFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    pj23119

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge