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LAMTOR1-APEX2
Plasmid#215556PurposeStable, inducible expression in lysosomeDepositorInsertLAMTOR1-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
5HT2A-APEX2
Plasmid#215558PurposeStable, inducible expression of 5HT2ADepositorInsertFlag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNAPf-BASU-His
Plasmid#218450PurposeE coli expression vector for SNAPf-BASU-His biotin ligaseDepositorInsertSNAPf-BASU-His fusion protein
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GFP-CAAX-he1.1:YFP
Plasmid#184816PurposeUsed to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic lineDepositorInsertQUAS:GFP-CAAX-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsGFP-CAAXExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDR274-gsc2-sgRNA
Plasmid#184818PurposeUsed to synthesize gRNA targeting exon 2 of the gsc2 geneDepositorInsertgsc2-sgRNA
UseCRISPR; Template for sgrna synthesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDestTol2-QUAS:NLS-mApple-he1.1:CFP
Plasmid#184814PurposeUsed to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic lineDepositorInsertQUAS:NLS-mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-mAppleExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-GFP-he1.1:CFP
Plasmid#184815PurposeUsed to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic lineDepositorInsertQUAS:NLS-GFP-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-GFPExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS18
Plasmid#218718PurposeExpresses N-terminally EGFP-tagged BBS18 in mammalian cellsDepositorInsertBBS18
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYBA4
Plasmid#217671PurposeFor expression of SUMO tagged human beta A4 crystallinDepositorInsertCRYBA4 (CRYBA4 Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterT7Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-LASV-GPC-HA-c3xFLAG
Plasmid#215691PurposeProduces the Lassa virus glycoprotein with a c-terminal HA tag and 3xFLAG tagDepositorInsertLASV GP-HA-FLAG
UseTagsHA-3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQFT-mNGsacB
Plasmid#203676PurposepQFT with mNeonGreen and sacB under control of PQJDepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Strep-TGT-His
Plasmid#206495PurposeExpresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His TagDepositorInsertE. coli tRNA Guanine Transglycosylase
UseTagsHis and StrepExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S1.46G>U
Plasmid#204160PurposepRegev_S1 with single mutation that disrupts secondary structure.DepositorInsertS1 exon with 46G>U substitution in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation46G>U substitutionPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc
Plasmid#199759PurposeElongation reporter construct to quantify elongation duration of 4_CGA stallingDepositorInsertsynYFP[TTG/AGA]-4xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-Green Falcan1
Plasmid#199705Purposegreen fluorescent indicator for cAMP imagingDepositorInsertGreen Falcan1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc
Plasmid#199760PurposeElongation reporter construct to quantify elongation duration of 6_CGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc
Plasmid#199761PurposeElongation reporter construct to quantify elongation duration of 6_AGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[AGA]-cLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN1-22xWmut_P
Plasmid#147237PurposeInsect Expression of CeAIN1-22xWmutDepositorInsertCeAIN1-22xWmut
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeNot2_P
Plasmid#147240PurposeInsect Expression of CeNot2DepositorInsertCeNot2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CePABP_569-646_P
Plasmid#147242PurposeInsect Expression of CePABP_569-646DepositorInsertCePABP_569-646
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1-22xWmut_P
Plasmid#147226PurposeInsect Expression of CeAIN1-22xWmutDepositorInsertCeAIN1-22xWmut
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CePABP_O
Plasmid#147148PurposeInsect Expression of CePABPDepositorInsertCePABP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1_O
Plasmid#147121PurposeInsect Expression of CeAIN1DepositorInsertCeAIN1 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN1_O
Plasmid#147130PurposeInsect Expression of CeAIN1DepositorInsertCeAIN1 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3A
Plasmid#198347PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3B
Plasmid#198348PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A-CC-Di
Plasmid#190707PurposeEncodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.DepositorInsertN-Venus-V150A-CC-Di
UseTagsExpressionMutationV150APromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A
Plasmid#190708PurposeControl plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.DepositorInsertN-Venus-V150A
UseTagsExpressionMutationV150APromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS myr-c-P3k
Plasmid#196373PurposeOncogenic with addition of a myristylation signal and expresses in avian cellsDepositorInsertMyr-c-P3k-FLAG
UseRetroviral; AvianTagsflag and myristoylationExpressionMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7PI-Sce ESARC D326A
Plasmid#195609PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT3593C, A3601C, T3602GPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgKcna2
Plasmid#192796PurposeEncodes SaCas9 and an sgRNA targeting mouse Kcna2DepositorInsertsgKcna2
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgKcna6
Plasmid#192797PurposeEncodes SaCas9 and an sgRNA targeting mouse Kcna6DepositorInsertsgKcna6
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_X5C_S277C
Plasmid#190177PurposeFor mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibilityDepositorInsertrat serotonin transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationCys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_Q1310A_right
Plasmid#187849Purposeexpresses the right-side half ND6-Q1310A in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_Q1310A_left
Plasmid#187848Purposeexpresses the left-side half ND6-Q1310A in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N(Q1310A)–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE
Plasmid#187419PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-66%GC-40bp
Plasmid#169200PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert66%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-20bp
Plasmid#169201PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-40bp
Plasmid#169202PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-100%GC-20bp
Plasmid#169203PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert100%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Left-half-120bp
Plasmid#169204PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Right-half-120bp
Plasmid#169205PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Left-half-120bp
Plasmid#169206PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50-0-50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Right-half-120bp
Plasmid#169207Purpose50-0-50%GC-Right-half-120bp-hairpinDepositorInsert50-0-50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-20bp
Plasmid#169196PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-40bp
Plasmid#169197PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only