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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,552 results for: eng

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Showing: 861 - 880 of 14552 results

  1. pcDNA3.1-hAsCpf1(TYCV) (pY210)

    Plasmid
    #89351
    Purpose
    Expresses humanized AsCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    S542R/K607R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89351-plasmid-map-sequence-id-178227

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  4. pCMV-PE6e

    Plasmid
    #207855
    Purpose
    Mammalian expression of PE6e prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6e
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, K918A, K775R)
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207855-plasmid-map-sequence-id-408162

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  11. pCMV-HF-BE3

    Plasmid
    #87439
    Purpose
    Expresses HF-BE3 with C-terminal NLS in mammalian cells
    Depositor
    David Liu
    Publication
    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HF-BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)
    Expression
    Mammalian
    Mutation
    Mammalian codon-optimized Cas9-HF
    Promoter
    CMV
    Available since
    June 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    87439-plasmid-map-sequence-id-298693

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  14. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87844-plasmid-map-sequence-id-174620

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  15. pcDNA3-CXCL12-sfGFP

    Plasmid
    #98961
    Purpose
    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCL12 (CXCL12 Human)
    Tags
    Superfolder GFP
    Expression
    Mammalian
    Mutation
    Full-length, wildtype
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98961-plasmid-map-sequence-id-193141

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Showing: 861 - 880 of 14552 results