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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgA2/κ

Plasmid

#61889

Purpose

Expresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgA2/κ

Plasmid

#61889

Purpose

Expresses HER2/neu receptor specific humanized IgA2/κ antibody isotype (Trastuzumab-IgA2/κ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-humanTRAF3 (1098)

Plasmid

#66927

Purpose

Mammalian Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-humanTRAF3 (1098)

Plasmid

#66927

Purpose

Mammalian Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLD1 translation factors

Plasmid

#117760

Purpose

BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 1-13

Use

Escherichia coli

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLD1 translation factors

Plasmid

#117760

Purpose

BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 1-13

Use

Escherichia coli

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Puro

Plasmid

#207726

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-ultraID-Puro

Plasmid

#207726

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_STRAP:1-350

Plasmid

#211139

Purpose

Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

STRAP:M1-A350 (STRAP Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_STRAP:1-350

Plasmid

#211139

Purpose

Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

STRAP:M1-A350 (STRAP Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWF079_LVt-EF1a-TCRGibson_humanTRAC

Plasmid

#233448

Purpose

Gibson assembly of TCRB-P2A-TCRa

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pWF079_LVt-EF1a-TCRGibson_humanTRAC

Plasmid

#233448

Purpose

Gibson assembly of TCRB-P2A-TCRa

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2

Plasmid

#44104

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2

Plasmid

#44104

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only