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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 37,603 results for: ELL

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Showing: 9081 - 9100 of 37603 results

  2. pcpBpaRS_v2

    Plasmid
    #196485
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Publication
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196485-plasmid-map-sequence-id-388866

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  6. alpha actinin delta ABD-GFP

    Plasmid
    #66935
    Purpose
    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted
    Depositor
    Pere Roca-Cusachs, Michael Sheetz
    Publication
    Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110
    Insert
    Alpha-actinin 1 (ACTN1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    actin binding domain (amino acids 30–253) is dele…
    Promoter
    CMV
    Available since
    June 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  7. MSP3-bio

    Plasmid
    #47731
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP3
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    47731-plasmid-map-sequence-id-68466

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  8. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)

    Viral prep
    #112677-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3-sACE2v2.4-IgG1

    Plasmid
    #149668
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    149668-plasmid-map-sequence-id-298411

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  10. pcDNA3-sACE2v2-sfGFP

    Plasmid
    #145173
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145173-plasmid-map-sequence-id-292589

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  12. aP2-GFPtpz

    Plasmid
    #114211
    Purpose
    aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage
    Depositor
    David Rowe
    Publication
    Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101
    Insert
    aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Mouse, Aequorea victoria, Synthetic)
    Tags
    None
    Expression
    Mammalian
    Promoter
    aP2/Fabp4
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114211-plasmid-map-sequence-id-220086

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  14. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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Showing: 9081 - 9100 of 37603 results