We narrowed to 4,257 results for: LRE

1. pRK5-FLAG-Sestrin2

   Plasmid

   #72595

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pRK5-HA-Sestrin2

   Plasmid

   #72593

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pMK293 (mAID-mCherry2-Hygro)

   Plasmid

   #72831

   Purpose

   C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mCherry2-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TORCAR(T/A)

   Plasmid

   #64928

   Purpose

   Negative-control mutant for TORCAR biosensor.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Mutation

   4EBP1 domain contains T37A and T46A mutations.

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-KIF1A

   Plasmid

   #172206

   Purpose

   Expresses KIF1A tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF1A (Kif1a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-eCas9

   Plasmid

   #140237

   Purpose

   Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a core

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF12J

   Plasmid

   #42943

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   EN+ Human L1 element (L1RE1 Human)
   

   Use

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   AgeI-BclI fragment swapped with nt 1927-3708 from…

   Promoter

   CMV MIE, L1 5'UTR

   Available since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

   Plasmid

   #86828

   Purpose

   Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. phage UbiC scAB-GFP

   Plasmid

   #104998

   Purpose

   Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   single chain Antibody against GCN4
   

   Use

   Lentiviral

   Tags

   GB1, HA tag, and sfGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2,0_3M5M_luciferase

    Plasmid

    #135475

    Purpose

    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, luciferase reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3_Sec61B-V5-APEX2

    Plasmid

    #83411

    Purpose

    Expressed C-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    Sec61B-V5-APEX2 (SEC61B Synthetic, Human)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-fDIO-oG-WPRE-SV40pA

    Plasmid

    #74291

    Purpose

    pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST17-His10-ccdB

    Plasmid

    #166145

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs-IP spGFP-ERGIC53

    Plasmid

    #38270

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Use

    Retroviral

    Tags

    EGFP with Calreticulin signal peptide at N-termin…

    Expression

    Mammalian

    Mutation

    deleted signal peptide (1-30 amino acids)

    Promoter

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-5X-1-Full-Length-MePCE

    Plasmid

    #113539

    Purpose

    Produces an N-terminal GST-tagged full length MePCE protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDuBir-Lbu-dCas13a-avitag

    Plasmid

    #100817

    Purpose

    Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

    Depositor

    Mitchell O'Connell

    Article

    Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

    Insert

    Lbu_Cas13a_R472A_H477A_R1048A_H1053A
    

    Use

    Tags

    AviTag and His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    T7

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-mAirn-R-loop

    Plasmid

    #134900

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mAirn promoter (Airn Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-hSNRPN-R-loop

    Plasmid

    #134902

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    hSNRPN promoter (SNRPN Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-FLEX-oG-WPRE-SV40pA

    Plasmid

    #74292

    Purpose

    pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1-a

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FgH1tUTG_mup53_Ex5

    Plasmid

    #85535

    Purpose

    Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAVTREGCaMP6f

    Plasmid

    #97410

    Purpose

    Amplified expression of GCaMP6f in combination with TET driver

    Depositor

    Tetsuo Yamamori

    Article

    Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3 CDK9 HA (P#635)

    Plasmid

    #14640

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 (CDK9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid

    #101299

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 and 698-906 of alpha-C…

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3_V5-APEX2-HMOX1

    Plasmid

    #85824

    Purpose

    Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    V5-APEX2-HMOX1 (HMOX1 Synthetic, Human)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMK294 (mAID-mCherry2-Bsr)

    Plasmid

    #72832

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-HA-ARHGAP29

    Plasmid

    #104154

    Purpose

    mammalian expression of HA tagged ARHGAP29

    Depositor

    Marius Sudol

    Article

    Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

    Insert

    ARHGAP29 (ARHGAP29 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMK291 (mAID-mClover-Bsr)

    Plasmid

    #72829

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Bsr
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge