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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

Plasmid

#153302

Purpose

Expresses ClpP protease in the cell-free TX-TL system

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

clpP (clpP Escherichia coli)

Expression

Bacterial

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-wildtype

Plasmid

#31972

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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SpySwitch

Plasmid

#184225

Purpose

Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpySwitch

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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mCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Pooled Library

#83996

Purpose

Version 2 of the mouse CRISPRa genome wide library

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
pBa.M6PR-GFP

Plasmid

#224407

Purpose

Mammalian expression of M6PR

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

M6PR (M6PR Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

Plasmid

#161580

Purpose

Express Kir2.1 in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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LV-PGK-Cre

Plasmid

#172433

Purpose

Lentivirus, expresses Cre recombinase

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

Cre

Use

Lentiviral

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_PEX14_Pex14

Plasmid

#178484

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PEX14_Pex14 (PEX14 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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ChromosomesMembranesandCytoskeleton

Plasmid

#62450

Purpose

MXS_chaining vector with 4 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-NLRP3

Plasmid

#73955

Purpose

Express EGFP-tagged human NLRP3 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pXN22-Dest

Plasmid

#105085

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG-3xHA

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

Plasmid

#160141

Purpose

Cre- dependent expression of Sun1GFP for nuclei isolation

Depositor

Andreas Pfenning

Article

Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

Insert

Sun1-GFP (Sun1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

N-truncated: removed amino acids 1-207, retained …

Promoter

Ef1a

Available Since

March 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Flag-SOD1-G93A

Plasmid

#235662

Purpose

Express human SOD1-G93A in mammalian cells

Depositor

Chen Seng Ng

Article

Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

Insert

SOD1-G93A (SOD1 Human)

Tags

Flag-tag at N-terminal

Expression

Mammalian

Mutation

changed Glycine 93 to alanine

Promoter

CMV promoter

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFL

Plasmid

#110739

Purpose

Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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HA-RNF34

Plasmid

#119938

Purpose

RING finger protein 34 expression (HA-tag at the N-terminus)

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Tags

HA

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only
pAAV CD68-hM4D(Gi)-mCherry (AAV9)

Viral Prep

#75033-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV CD68-hM4D(Gi)-mCherry (#75033). In addition to the viral particles, you will also receive purified pAAV CD68-hM4D(Gi)-mCherry plasmid DNA. CD68-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced microglial inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Article

Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Promoter

CD68

Tags

mCherry

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only
mEos2-ZO1-C-14

Plasmid

#57440

Purpose

Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

ZO1 (TJP1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

Plasmid

#183775

Purpose

AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

NLS-GFP

Use

AAV

Mutation

NA

Promoter

CAG

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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