182,051 results
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Plasmid#51271PurposeComponent of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)DepositorPublicationInsertNLS-HA-Bxb1
ExpressionMammalianPromoterCAGAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (AAV1)
Viral prep#51509-AAV1PurposeReady-to-use AAV1 particles produced from AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (#51509). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE plasmid DNA. hSyn-driven, Cre-dependent expression of cytoplasmic tdTomato and presynaptic EGFP (synaptophysin-fused). These AAV preparations are suitable purity for injection into animals.DepositorPromoterphSyn1TagstdTomato (cytoplasmic, Cre-dependent), EGFP (presynaptic, C…Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH
Plasmid kit#1000000163PurposeBRET biosensor plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation.DepositorApplicationGene Expression and LabelingVector typeMammalian ExpressionAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-FB-dCas9-puro
Plasmid#100547PurposeExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsN-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin
UseCRISPRTagsFLAG, Biotin-acceptor-site (Avitag)ExpressionMammalianPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Apple-53BP1trunc
Plasmid#69531PurposeFluorescent reporter for DNA damageDepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsApple fluorescent proteinExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
7M8
Plasmid#64839PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.DepositorInsert7M8 cap
UseAAVMutation7mer insertion in the AAV2 capAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His6 GST TEV LIC cloning vector (1G)
Plasmid#29655DepositorTypeEmpty backboneTags6xHis, GST, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-2xFLAG-SREBP-1c
Plasmid#26802DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSREBP-1c (SREBF1 Human)
Tags2XFLAGExpressionMammalianAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1477 pcDNA3 flag HA Akt1
Plasmid#9021DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt1 (AKT1 Human)
TagsHA and flagExpressionMammalianAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV2/rh10
Plasmid#112866PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap-rh10
UseAAVPromoterTruncated P5Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_BE4max
Plasmid#112093PurposeC:G-to-T:A base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKD3
Plasmid#45604DepositorInsertFRT-cat-FRT
ExpressionBacterialPromoterR6KAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-flag YAP2 5SA
Plasmid#27371DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYAP2 (YAP1 Human)
TagsflagExpressionMammalianMutationSerines at positions 61, 109, 127, 128, 131, 163,…Available sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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mRFP-Rab7
Plasmid#14436PurposeMammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab 7 (RAB7A Canis familialis)
TagsmRFPExpressionMammalianMutation3 silent mutations near the C-terminusAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HDAC7 Flag
Plasmid#13824PurposeMammalian expression of human histone deacetylase 7 with flag tagDepositorAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV5B-Flag-Smad3
Plasmid#11742DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMAD3 (SMAD3 Human)
TagsFlagExpressionMammalianAvailable sinceAug. 7, 2006AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pGP-AAV-syn-jGCaMP8s-WPRE (AAV1)
Viral prep#162374-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only