We narrowed to 32,043 results for: fer

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pEGB 35S:Renilla:Tnos (GB0109)

Plasmid

#68215

Purpose

Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP

Plasmid

#166439

Purpose

Bacterial expression of 6x His tagged mEGFP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP

Expression

Bacterial

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Cyclin D1

Plasmid

#19927

Depositor

Yue Xiong

Article

Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

Insert

Cyclin D1 (CCND1 Human)

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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EC2_10_dCas9_VPR_HC_sgRNA

Plasmid

#163712

Purpose

Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-SopE

Plasmid

#221327

Purpose

Dox-inducible expression of SopE in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

SopE

Use

Retroviral

Tags

FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PIPK1 gamma 90

Plasmid

#22299

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Relb

Plasmid

#139810

Purpose

doxycycline-inducible expression of mouse Relb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Relb (Relb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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bdSUMO-GFP-HiBit

Plasmid

#170989

Purpose

For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pV1382

Plasmid

#111436

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-P2A-APEX2-VHH[GFP]

Plasmid

#126749

Purpose

Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

VHH[EGFP]

Use

Zebrafish muscle expression clone

Tags

mKate2-P2A-APEX2

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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GOLPH3 shRNA

Plasmid

#163860

Purpose

shRNA targeting the coding sequences of GOLPH3

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

golgi phosphoprotein 3 (GOLPH3 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pIS1-Eef25UTR-renilla

Plasmid

#38235

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Eef2 5'UTR / Renilla (Eef2 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Eef2 5' UTR fused to renilla ORF

Promoter

Endogenous Eef2

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-V106W

Plasmid

#215827

Purpose

Express CBE6d V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Raichu-Rab5/PM

Plasmid

#214819

Purpose

Encoding a Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.

Expression

Mammalian

Promoter

EF-1α

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pACYC-yhaM-RFP

Plasmid

#207651

Purpose

Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-Cre

Plasmid

#50935

Purpose

retroviral expression of Cre recombinase

Depositor

Pier Pandolfi

Article

Chen et al Nature. 2005 Aug 4;436(7051):725-30.

Insert

Cre recombinase

Use

Cre/Lox and Retroviral

Tags

NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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RC(WW)-LgBit

Plasmid

#208931

Purpose

Recognition domain for in vitro MAPK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

WW domain of PIN1 (PIN1 Human)

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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