We narrowed to 13,693 results for: crispr cas9

1. pBWB419

   Plasmid

   #140637

   Purpose

   RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGM51559

   Plasmid

   #153226

   Purpose

   Control plasmid for knockout of TRY and CPC genes

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Cas9, guide RNA for AP3, FAST marker
   

   Use

   Synthetic Biology

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGM55361

   Plasmid

   #153227

   Purpose

   Control plasmid for knockout of AP3 gene

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Cas9, guide RNA for TRY and CPC and FAST marker
   

   Use

   Synthetic Biology

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWR58

   Plasmid

   #202795

   Purpose

   integrating bioluminescene reporter for Cas9 activity in S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   P-TDH3/coCBG(first portion)-YUM1
   P-TEF1/ShBle/T-ACT1
   YUM1-coCBG(last portion)/T-ADH2
   URA3 and flanking sequence
   

   Expression

   Yeast

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGM41991

   Plasmid

   #153219

   Purpose

   Level M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDsRed-attP

   Plasmid

   #51019

   Purpose

   Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

   Depositor

   Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Inserts

   attP
   LoxP-3xP3-DsRed-LoxP
   

   Use

   High copy, amp resistance

   Promoter

   3xP3 and None

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-TLR targeting vector

   Plasmid

   #64215

   Purpose

   Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Insert

   traffic light reporter (TLR) (AAVS1 Human)
   

   Use

   Human targeting

   Mutation

   see publication for details

   Promoter

   CAG

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE1

   Plasmid

   #132774

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE1
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. dxCas(3.7)-VPR

   Plasmid

   #108383

   Purpose

   Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.7)-VPR
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH-ABE8e

    Plasmid

    #200774

    Purpose

    For plant adenine base editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

    Insert

    nCas9(D10A)-TadA8e
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

    Promoter

    maize Ubiquitin-1, OsU3

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Neo-CAG-Flpe-ERT2

    Plasmid

    #68460

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE unc13a GFP KI

    Plasmid

    #131498

    Purpose

    Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Unc13a Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6 SgRNA RBM20

    Plasmid

    #162735

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    Spacer sequence for RBM20
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2-LyzC-RacFRET-U6ac-ctrl guides

    Plasmid

    #168247

    Purpose

    "label active Rac; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    RacFRET
    

    Use

    Zebrafish expression

    Promoter

    LyzC

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-tLuc-mCherry

    Plasmid

    #107553

    Purpose

    tLuc-mCherry in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. dxCas(3.6)-VPR

    Plasmid

    #108388

    Purpose

    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.6)-VPR
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ScFv-2ERT2-VPH

    Plasmid

    #120556

    Purpose

    Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO

    Plasmid

    #155048

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PAX6 CKO 2/7 Donor plasmid

    Plasmid

    #68467

    Purpose

    donor plasmid for targeting PAX6 locus

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    FRT flanked exon4 of PAX6 gene (PAX6 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    mouse PGK promoter

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6 SgRNA GAPDH

    Plasmid

    #162736

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    Spacer sequence for GAPDH
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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