166,542 results
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Plasmid#213520PurposeExpresses NSP14 of hCoV NL63DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only
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pRSF_his6_sumo_229E_NSP14
Plasmid#213521PurposeExpresses NSP14 of hCoV 229EDepositorInserthCoV 229E NSP14
UseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-TID-FKBP-HA3
Plasmid#227998PurposeExpresses TurboID-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Zeo
Plasmid#227271PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdOm2.1
Plasmid#210278PurposeEmpty backbone with ampicillin resistance for high expression in mammalian cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK484 (2mAID-Hygro)
Plasmid#214396Purpose2mAID for N-terminal taggingDepositorInsert2mAID-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK487 (Hygro-P2A-3mAID)
Plasmid#214399Purpose3mAID for C-terminal taggingDepositorInsertHygro-P2A-3mAID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK450 (BromoTag-mCherry-BSD)
Plasmid#214380PurposeBromoTag-mCherry for N-terminal taggingDepositorInsertBromoTag-mCherry-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A
Plasmid#169826PurposeExpresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation siteDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationT456A S1406A S1859A; TCCC -> AGTC silent mutat…PromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR074-control-sfGFP
Plasmid#185032PurposeJ23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationNucleotides of rpmE 5'UTR scrambled, Only fi…PromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KMT2A_AFDN
Plasmid#205840PurposeExpress mEGFP-tagged fusion protein, KMT2A_AFDN from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-miR-503-5p
Plasmid#135725PurposeMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-503-5p)DepositorInsertmiR-503-5p reverse complement
UseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag VRK3
Plasmid#20664DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV PHP.S)
Viral Prep#28306-PHP.SPurposeReady-to-use AAV PHP.S particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV were produced with the PHP.S serotype, which permits efficient transduction of the peripheral nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LC3-RFP-LC3ΔG
Plasmid#168997PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-hTERT-IRES-hygro
Plasmid#85140PurposeLentiviral expression of hTERT, used to create immortalized cell linesDepositorAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
sortase A pentamutant (eSrtA) in pET29
Plasmid#75144Purposeevolved sortase A (eSrtA) pentamutant with improved kinetics and activityDepositorInsertsortase A pentamutant
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196T. Deleted amino a…PromoterT7Available SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mCherry-EGFP-LC3B
Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)
Viral Prep#73179-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.DepositorPromoterTagsNoneAvailable SinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2GFP
Plasmid#82416Purpose3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning siteDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFS (P2A)Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only