165,637 results

1. pMRS-XylS-dCas9

   Plasmid

   #220196

   Purpose

   mini-Tn7 dCas9 expression vector - XylS/pM promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   XylS/pM

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YFP-CL1

   Plasmid

   #11950

   Depositor

   Nico Dantuma

   Article

   Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

   Insert

   YFP-CL1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DRH337: AAV-hsyn-CheRiff-eGFP

   Plasmid

   #51697

   Purpose

   AAV production of CheRiff channelrhodopsin actuator

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   CheRiff-eGFP
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   hSynapsin

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. iBE4max YE1 NG CLYBL

   Plasmid

   #174570

   Purpose

   doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet ON

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgTP53_3

   Plasmid

   #78164

   Purpose

   sgTP53

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. L4440_BioBrick-SCR-sgRNA-A

   Plasmid

   #177811

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CP1041-ABE8e

   Plasmid

   #138493

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1041)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMRS-LacI-GFP

   Plasmid

   #220198

   Purpose

   mini-Tn7 GFP expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   monomeric superfolder green fluorescent protein
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   LacI/pLac

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOpen-DNA Topoisomerase 1B Vaccinia Virus

    Plasmid

    #165510

    Purpose

    Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    DNA Topoisomerase 1B Vaccinia Virus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. CMVTO-Furin

    Plasmid

    #179920

    Purpose

    Furin protein

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    Furin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-GFP-CD63

    Plasmid

    #194989

    Purpose

    overexpress CD63

    Depositor

    Angelique Bordey

    Insert

    GFP-CD63
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mc2 mNeon

    Plasmid

    #206218

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1_T6B -mCherry

    Plasmid

    #205600

    Purpose

    expresses human T6B peptide fused with mChery in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pgRNA-modified

    Plasmid

    #84477

    Purpose

    guide RNA expression lentiviral construct

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    guide RNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mBFP-APP-mGFP

    Plasmid

    #196694

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mEGFP and mtagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW57.1-DUX4-CA

    Plasmid

    #99281

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (codon altered) (DUX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOpen-P45

    Plasmid

    #165570

    Purpose

    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    P45
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDESTmycDICER

    Plasmid

    #19873

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    dicer 1, ribonuclease type III (DICER1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMOD_A1110

    Plasmid

    #91031

    Purpose

    Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG2-EGFP-C-IFT144

    Plasmid

    #218740

    Purpose

    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT144 (WDR19 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. L4440_BioBrick-SCR-sgRNA-B

    Plasmid

    #177812

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    Scrambled sgRNA targeting sequences
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF0209

    Plasmid

    #141556

    Purpose

    Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Mouse Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187081

    Purpose

    Lentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expression

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    Lentiviral

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

26. pACYC-Skp-TF

    Plasmid

    #83921

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Trigger Factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YFP NLS Beta-Actin

    Plasmid

    #60613

    Purpose

    Encodes YFP and NLS tagged beta-actin

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. dCas9-5xGCN4-P2A-BFP

    Plasmid

    #184438

    Purpose

    dCas9 fused with 5 copies of GCN4 epitope

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    dCas9-5xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAX APP C99

    Plasmid

    #30146

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP C99 (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal from BACE site of APP

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. VE-cad-eGFP Donor plasmid

    Plasmid

    #92309

    Purpose

    Knock eGFP into human VE-cad locus

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad Homologous Recombination Arms (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    about 30 a.a. truncated at end of protein

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge