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pcDNA3-ATR WT

Plasmid

#31611

Depositor

Aziz Sancar

Article

Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87828

Purpose

High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87828

Purpose

High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-CBE

Plasmid

#196293

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding CBE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pM-CBE

Plasmid

#196293

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding CBE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-mCherry-FLAG-CD59-GPI

Plasmid

#50378

Purpose

Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

mCherry

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pME-mCherry-FLAG-CD59-GPI

Plasmid

#50378

Purpose

Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Article

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Insert

mCherry

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
DsRed2-pBAD

Plasmid

#54608

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and DsRed2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
DsRed2-pBAD

Plasmid

#54608

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and DsRed2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-hSyn-EGFP

Plasmid

#114213

Purpose

AAV vector (control) that targets expression of EGFP to neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-hSyn-EGFP

Plasmid

#114213

Purpose

AAV vector (control) that targets expression of EGFP to neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX-CMV-SP-YFP-STM2(15-746)

Plasmid

#18862

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 2 (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

YFP inserted between signal peptide(SP) of STIM1 …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX-CMV-SP-YFP-STM2(15-746)

Plasmid

#18862

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 2 (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

YFP inserted between signal peptide(SP) of STIM1 …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
N1-mSin1.1-GFP

Plasmid

#72907

Purpose

GFP-tagged mSin1 (isoform 1)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

mSin1.1 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
N1-mSin1.1-GFP

Plasmid

#72907

Purpose

GFP-tagged mSin1 (isoform 1)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

mSin1.1 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-N-Flag-NLRP3 91-710

Plasmid

#75140

Purpose

Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 91-710

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-N-Flag-NLRP3 91-710

Plasmid

#75140

Purpose

Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 91-710

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3β-FLAG-CBP-LD-HA

Plasmid

#32906

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

C/EBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

L1435A/D1436A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3β-FLAG-CBP-LD-HA

Plasmid

#32906

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

C/EBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

L1435A/D1436A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
mOrange-pBAD

Plasmid

#54751

Purpose

Localization: Protein Expression Vector , Excitation: 548, Emission: 562

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and mOrange

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mOrange-pBAD

Plasmid

#54751

Purpose

Localization: Protein Expression Vector , Excitation: 548, Emission: 562

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and mOrange

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3A-eBE-Y130F

Plasmid

#113423

Purpose

Expresses hA3A-eBE-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3A-eBE-Y130F

Plasmid

#113423

Purpose

Expresses hA3A-eBE-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-BH

Plasmid

#60499

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-BH

Plasmid

#60499

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDIA

Plasmid

#51625

Purpose

E. coli genome modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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41,440 results