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pLKO-puro-tetON-shOGDH 2956

Plasmid

#110943

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

Lentiviral and RNAi

Promoter

H1/TO

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSlick-Neo-RECK

Plasmid

#121986

Purpose

Tetracycline-inducible expression of untagged full-length RECK

Depositor

GuangJun Zhang

Article

Kumari et al Oncotarget. 2018 May 4;9(34):23494-23504

Insert

RECK (RECK Human)

Use

Lentiviral

Promoter

Tight TRE promoter

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

Plasmid

#128165

Purpose

UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).

Depositor

Marcus Noyes

Article

Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

Insert

10 amino acid glycine/serine linker + 5alanines

Use

Synthetic Biology

Tags

FLAG and zif268

Expression

Bacterial

Promoter

UV5

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 (delta110)

Plasmid

#71861

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, missing first 110 amino acids

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-miRFP-6xFLAG

Plasmid

#161614

Purpose

Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

miRFP-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A T31A-NG-nCas9

Plasmid

#163539

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3 2 kb prom. and exon 1 fragment CD274

Plasmid

#107007

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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OA-1136J

Plasmid

#153023

Purpose

Express His-MBP-TEV-CasRx recombinant protein

Depositor

Omar Akbari

Article

Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

Insert

CasRx

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT6(V86K/D88A)

Plasmid

#164698

Purpose

Expression of PRMT6(V86K/D88A) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

Insert

PRMT6 (PRMT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

V86K, D88A

Promoter

CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-mKdm2b (del CxxC domain)-Flag

Plasmid

#61740

Purpose

Express CxxC domain deleted mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Tags

Flag

Mutation

delete aa 588-624

Promoter

EF1a

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-TRE-FH-TET3 short

Plasmid

#166916

Purpose

Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

TET3 short isoform

Use

Lentiviral

Tags

HA Tag

Expression

Mammalian

Mutation

Changed nucleotide 18 (G) to (A) to delete FseI r…

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10

Plasmid

#162385

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-jYCaMP1s

Plasmid

#135424

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has Service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_12E

Plasmid

#98654

Purpose

Expresses MBP-tagged phosphomimetic FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 (1-119aa)

Plasmid

#71862

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, first 119 amino acids

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_SOX2

Plasmid

#45270

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MCOPS3 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

Plasmid

#163572

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD9

Plasmid

#195847

Purpose

Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD9 (FZD9 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA3

Plasmid

#166915

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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