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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,383 results for: mpl

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Showing: 1651 - 1680 of 11383 results

  3. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  4. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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  9. pX459-sgAAVS1

    Plasmid
    #184403
    Purpose
    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    AAVS1-specific sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184403-plasmid-map-sequence-id-362381

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  10. G protein alpha-q-YFP

    Plasmid
    #55782
    Purpose
    This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-q EE YFP (Gnaq Mouse)
    Tags
    The EE epitope was introduced into the alpha-q se…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    55782-plasmid-map-sequence-id-119295

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  15. DDX41-GFP

    Plasmid
    #175494
    Purpose
    Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.
    Depositor
    Evan Braunstein
    Article
    Li et al Haematologica. 2016 Jun;101(6):e228-31.
    Insert
    DDX41 (DDX41 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175494-plasmid-map-sequence-id-345045

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Showing: 1651 - 1680 of 11383 results