We narrowed to 75,511 results for: rest

1. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

   Plasmid

   #73542

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

   Plasmid

   #73543

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. L1-AA027

   Plasmid

   #185752

   Purpose

   Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

   Depositor

   Peter Szoevenyi

   Article

   Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

   Insert

   HygR2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIIS18-BufAI-2

   Plasmid

   #128651

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIIS18-BufAI-1

   Plasmid

   #128650

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTK-EdnrbE2-Cerulean

   Plasmid

   #127771

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE2
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTK-EdnrbE1-Citrine

   Plasmid

   #127773

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE1
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-Sox3gRNA-T2

   Plasmid

   #127778

   Purpose

   Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox3gRNAT2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX 4T-1/MKRN1

    Plasmid

    #78757

    Purpose

    To purify GST-tagged MKRN1 recombinant protein

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-MKRN1 110-482

    Plasmid

    #78747

    Purpose

    To overexpress MKRN1 110-482 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    110-482

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HA-MKRN1 1-280

    Plasmid

    #78750

    Purpose

    To overexpress -MKRN1 1-280 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    1-280

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBGSA FPR1 N297A

    Plasmid

    #66723

    Purpose

    Constitutive expression of FPR1 N297A in mammalian cells.

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

    Insert

    FPR1 (haplotype 3) (FPR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N297A

    Promoter

    SR-alpha

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-myc-Rem2 S334A

    Plasmid

    #51593

    Purpose

    expresses RNAiR myc-tagged Rem2 S-A mutant

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

    Insert

    Rem2 (Rem2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Serine 334 to Alanine; also RNAi resistant

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pUC:FCP:ShBle:FCP:EGFP

    Plasmid

    #85987

    Purpose

    Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

    Depositor

    Thomas Mock

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Inserts

    ShBle
    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    opposing T7 promoters

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIV-D3psi/delta env/delta Vif/delta Vpr

    Plasmid

    #132928

    Purpose

    Production of SIV-VLPs containing Vpx protein

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    Gag, pol, and vpx
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3b-8XGliBS:EGFP

    Plasmid

    #84602

    Purpose

    Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

    Depositor

    James Chen

    Article

    Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    8X Gli-binding site

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTrichoGate-16

    Plasmid

    #160716

    Purpose

    T-nos Terminator bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    nopaline synthase terminator (T-nos) bordered by BsaI restriction sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Incorporation of BsaI restriction sites

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-TetOne-Puro-mitoLbNOX-FLAG

    Plasmid

    #218667

    Purpose

    pLVX-TetOne-Puro-mitoLbNOX-FLAG

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoLbNOX
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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